J 1999

Sequence-dependent conformational changes in DNA induced by polynuclear platinum complexes

MCGREGOR, Tracey, Zdeňka BALCAROVÁ, Yun QU, My-Chau TRAN, Renata ŽALUDOVÁ et. al.

Basic information

Original name

Sequence-dependent conformational changes in DNA induced by polynuclear platinum complexes

Authors

MCGREGOR, Tracey (840 United States of America), Zdeňka BALCAROVÁ (203 Czech Republic), Yun QU (840 United States of America), My-Chau TRAN (840 United States of America), Renata ŽALUDOVÁ (203 Czech Republic), Viktor BRABEC (203 Czech Republic) and Nicholas FARRELL (840 United States of America, guarantor)

Edition

JOURNAL OF INORGANIC BIOCHEMISTRY, 1999, 0162-0134

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.463

Organization unit

Faculty of Science

Keywords in English

DNA; polynuclear platinum complexes
Změněno: 5/8/2010 13:37, RNDr. Kamila Neplechová, Ph.D.

Abstract

V originále

In this work, the B to Z transition of poly(dGdC).poly(dGdC) and the B to A transition of poly(dG).poly(dC) and of calf thymus (CT) DNA fragments modified by antitumor bifunctional polynuclear platinum complexes were investigated by circular dichroism (CD). The transition from the B to Z form of DNA was inducible with all three compounds studied, as indicated by an inversion of the B form spectra. The B to A transition in poly(dG) poly(dC) was induced easily by platinum complex binding alone, while the B to A transition in CT DNA was induced by ethanol but inhibited by coordination of all polynuclear platinum compounds used here. It was shown that the compound [(cisPtCl(NH3)(2))(2) mu(H2N(CH2)(6)NH2)] (NO3)(2) (1,1/c,c) was most effective at inhibiting the B to A transition in CT DNA, and [(transPtCl(NH3)(2))(,2 mu)(trans- Pt(NH3)(2)(H2N(CH2)(6)NH2)(2))](NO3)(4) (1,0,1/t,t,t) was least effective, while the effectiveness of [(transPtCl(NH3)(2))(,2) mu(H,2N(CH2)(6)NH2)] (NO3)(2) (1,1/t,t) fell between.

Links

GA305/99/0695, research and development project
Name: Ovlivnění konformace DNA protinádorově účinnými komplexy kovů. Vztah k vývoji nových cytostatik.