DNA interactions of bifunctional dinuclear platinum(II)
antitumor agents

J 1997

DNA interactions of bifunctional dinuclear platinum(II) antitumor agents

ŽALUDOVÁ, Renata, Alena ŽÁKOVSKÁ, Jana KAŠPÁRKOVÁ, Zdeňka BALCAROVÁ, Vladimír KLEINWÄCHTER et. al.

Basic information

Original name

DNA interactions of bifunctional dinuclear platinum(II) antitumor agents

Authors

ŽALUDOVÁ, Renata (203 Czech Republic), Alena ŽÁKOVSKÁ (203 Czech Republic), Jana KAŠPÁRKOVÁ (203 Czech Republic), Zdeňka BALCAROVÁ (203 Czech Republic), Vladimír KLEINWÄCHTER (203 Czech Republic), Oldřich VRÁNA (203 Czech Republic), Nicholas FARRELL (840 United States of America, guarantor) and Viktor BRABEC (203 Czech Republic)

Edition

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 0014-2956

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.136

Organization unit

Faculty of Science

Keywords in English

DNA; platinum drug; dinuclear complex; cross-link; anticancer effect

Změněno: 5/8/2010 14:29, RNDr. Kamila Neplechová, Ph.D.

Abstract

V originále

Modifications of natural DNA in a cell-free medium by dinuclear bisplatinum complexes with equivalent coordination spheres, represented by the general formula [{trans-PtCl(NH3)(2)}(2)(H2N-R-NH2)](2+), where R is a propane or hexane, were studied by various methods of biochemical analysis or molecular biophysics. These methods include binding studies by means of differential-pulse polarography, measurements of melting curves with the aid of absorption spectrophotometry, measurements of CD spectra, ELISA with specific antibodies that recognize DNA modified by platinum complexes, interstrand crosslinking assay employing gel electrophoresis under denaturing conditions and mapping of DNA adducts by means of transcription assays. The results indicated that the major adduct of [{trans-PtCl(NH3)(2)}(2)(H2N-R-NH2)](2+) in DNA was an interstrand cross-link which was formed with a relatively short half-time (approximate to 1 h). At least some types of these interstrand cross-links induced local denaturational changes in the DNA.

Links

GA307/97/P029, research and development project

Name: Molekulární mechanismus působení protinádorově účinných bis platinových komplexů

Citovat

ŽALUDOVÁ, Renata, Alena ŽÁKOVSKÁ, Jana KAŠPÁRKOVÁ, Zdeňka BALCAROVÁ, Vladimír KLEINWÄCHTER, Oldřich VRÁNA, Nicholas FARRELL and Viktor BRABEC. DNA interactions of bifunctional dinuclear platinum(II) antitumor agents. EUROPEAN JOURNAL OF BIOCHEMISTRY. 1997, vol. 246, No 2, 10 pp. ISSN 0014-2956.

@article{893205,
   author = {Žaludová, Renata and Žákovská, Alena and Kašpárková, Jana and Balcarová, Zdeňka and Kleinwächter, Vladimír and Vrána, Oldřich and Farrell, Nicholas and Brabec, Viktor},
   article_number = {2},
   keywords = {DNA; platinum drug; dinuclear complex; cross-link; anticancer effect},
   language = {eng},
   issn = {0014-2956},
   journal = {EUROPEAN JOURNAL OF BIOCHEMISTRY},
   title = {DNA interactions of bifunctional dinuclear platinum(II) antitumor agents},
   volume = {246},
   year = {1997}
}

TY  - JOUR
ID  - 893205
AU  - Žaludová, Renata - Žákovská, Alena - Kašpárková, Jana - Balcarová, Zdeňka - Kleinwächter, Vladimír - Vrána, Oldřich - Farrell, Nicholas - Brabec, Viktor
PY  - 1997
TI  - DNA interactions of bifunctional dinuclear platinum(II) antitumor agents
JF  - EUROPEAN JOURNAL OF BIOCHEMISTRY
VL  - 246
IS  - 2
SN  - 00142956
KW  - DNA
KW  - platinum drug
KW  - dinuclear complex
KW  - cross-link
KW  - anticancer effect
N2  - Modifications of natural DNA in a cell-free medium by dinuclear bisplatinum complexes with equivalent coordination spheres, represented by the general formula [{trans-PtCl(NH3)(2)}(2)(H2N-R-NH2)](2+), where R is a propane or hexane, were studied by various methods of biochemical analysis or molecular biophysics. These methods include binding studies by means of differential-pulse polarography, measurements of melting curves with the aid of absorption spectrophotometry, measurements of CD spectra, ELISA with specific antibodies that recognize DNA modified by platinum complexes, interstrand crosslinking assay employing gel electrophoresis under denaturing conditions and mapping of DNA adducts by means of transcription assays. The results indicated that the major adduct of [{trans-PtCl(NH3)(2)}(2)(H2N-R-NH2)](2+) in DNA was an interstrand cross-link which was formed with a relatively short half-time (approximate to 1 h). At least some types of these interstrand cross-links induced local denaturational changes in the DNA.
ER  -

ŽALUDOVÁ, Renata, Alena ŽÁKOVSKÁ, Jana KAŠPÁRKOVÁ, Zdeňka BALCAROVÁ, Vladimír KLEINWÄCHTER, Oldřich VRÁNA, Nicholas FARRELL and Viktor BRABEC. DNA interactions of bifunctional dinuclear platinum(II) antitumor agents. \textit{EUROPEAN JOURNAL OF BIOCHEMISTRY}. 1997, vol.~246, No~2, 10 pp. ISSN~0014-2956.

Detailed Information on Publication Record