RESTRICTION-ENZYME CLEAVAGE OF DNA MODIFIED BY PLATINUM(II) COMPLEXES

BRABEC, Viktor and Zdeňka BALCAROVÁ. RESTRICTION-ENZYME CLEAVAGE OF DNA MODIFIED BY PLATINUM(II) COMPLEXES. EUROPEAN JOURNAL OF BIOCHEMISTRY. 1993, vol. 216, No 1, 5 pp. ISSN 0014-2956.

Basic information

Original name

RESTRICTION-ENZYME CLEAVAGE OF DNA MODIFIED BY PLATINUM(II) COMPLEXES

Authors

BRABEC, Viktor and Zdeňka BALCAROVÁ

Edition

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 0014-2956

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II); ENDONUCLEASE ECORV; SUPERCOILED DNA; CROSS-LINKS; BINDING; ADDUCTS; INHIBITION; PBR322; TRANS-DIAMMINEDICHLOROPLATINUM(II); CIS-DICHLORODIAMMINEPLATINUM(II)

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Abstract

V originále

The effect of binding of cis-diamminedichloroplatinum(II), its trans isomer and diethylenetriaminechloroplatinum(II) chloride to DNA on the splicing effectiveness of BamHI, EcoRI and SalI restriction endonucleases has been determined by means of gel electrophoresis. All three platinum complexes inhibit the cleavage of linearized plasmid DNA. In addition, the three platinum complexes bound to DNA constitute a barrier across which the linear diffusion of EcoRI on DNA is difficult. We interprete these findings to mean that the splicing effectiveness of restriction enzymes is influenced by bifunctional and monofunctional DNA adducts of platinum via both steric interference and DNA conformational distortions. Whereas the platinum adducts in the restriction sites or in their very close proximity inhibit the cleavage, the lesions occurring a greater distance from the restriction site can slow down the process of the localization of recognition sequences.