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@proceedings{893717,
author = {Masařík, Michal and Gumulec, Jaromír and Kuchtíčková, Šárka and Kudláčková, Veronika and Jurajda, Michal and Pavlík, Dušan and Rovný, Arne and Hrabec, Roman and Křížková, Soňa and Kizek, René},
booktitle = {Febs Journal},
keywords = {prostate carcinoma;metallothionein;amacr;tumor marker;},
language = {eng},
title = {Determination of novel tumor markers in prostate carcinoma},
url = {http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2010.07680.x/pdf},
year = {2010}
}
TY - CONF
ID - 893717
AU - Masařík, Michal - Gumulec, Jaromír - Kuchtíčková, Šárka - Kudláčková, Veronika - Jurajda, Michal - Pavlík, Dušan - Rovný, Arne - Hrabec, Roman - Křížková, Soňa - Kizek, René
PY - 2010
TI - Determination of novel tumor markers in prostate carcinoma
KW - prostate carcinoma;metallothionein;amacr;tumor marker;
UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2010.07680.x/pdf
N2 - Prostate cancer is second leading cause of death among men. Metallothionein (MT) and alpha-methylacyl-CoA (AMACR) racemase could be such markers. Expression of bothof these proteins is highly unregulated in prostate cancer. MT levels are elevated most likely due to disbalance of zinc metabolism in this disease, and reason of elevation of AMACR is still unknown. In our work we analyzed levels of MT, PSA and AMACR in cell lines derived from prostate carcinoma - LNCaP, PC-3 and 22RVL, which were compared to prostate cell line PNT1A derived from normal prostate epithelium. For analysis we used immunoseparation techniques, western blotting and SDS-PAGE. In addition to this clasical methods of molecular biology we used novel electrochemical detection and capillary electrophoresis on the chip.
ER -
MASAŘÍK, Michal, Jaromír GUMULEC, Šárka KUCHTÍČKOVÁ, Veronika KUDLÁČKOVÁ, Michal JURAJDA, Dušan PAVLÍK, Arne ROVNÝ, Roman HRABEC, Soňa KŘÍŽKOVÁ a René KIZEK. Determination of novel tumor markers in prostate carcinoma. In \textit{Febs Journal}. 2010. ISSN~1742-4658.
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