Experimental Methods for Simultaneous Measurement of Action
Potentials and Electrograms in Isolated Heart

J 2010

Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart

KOLÁŘOVÁ, Jana, Kateřina FIALOVÁ, Oto JANOUŠEK, Marie NOVÁKOVÁ, Ivo PROVAZNÍK et. al.

Základní údaje

Originální název

Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart

Autoři

KOLÁŘOVÁ, Jana (203 Česká republika, garant), Kateřina FIALOVÁ (203 Česká republika), Oto JANOUŠEK (203 Česká republika), Marie NOVÁKOVÁ (203 Česká republika) a Ivo PROVAZNÍK (203 Česká republika)

Vydání

Physiological Research, 2010, 0862-8408

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.646

Kód RIV

RIV/00216224:14110/10:00044615

Organizační jednotka

Lékařská fakulta

UT WoS

000279049100011

Klíčová slova česky

Napěťově citlivá barviva; Pohybový artefakt; Ischemie; Vápník

Klíčová slova anglicky

Voltage-Sensitive Dye; Motion Artifact; Ischemia; Calcium

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 10. 2010 09:47, prof. MUDr. Marie Nováková, Ph.D.

Anotace

V originále

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.

Návaznosti

GA102/07/1473, projekt VaV

Název: Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací

Investor: Grantová agentura ČR, Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací

MSM0021622402, záměr

Název: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časná diagnostika a léčba kardiovaskulárních chorob

Citovat

KOLÁŘOVÁ, Jana, Kateřina FIALOVÁ, Oto JANOUŠEK, Marie NOVÁKOVÁ a Ivo PROVAZNÍK. Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart. Physiological Research. 2010, vol. 59, Suppl 1, s. S71-S80, 10 s. ISSN 0862-8408.

@article{895673,
   author = {Kolářová, Jana and Fialová, Kateřina and Janoušek, Oto and Nováková, Marie and Provazník, Ivo},
   article_number = {Suppl 1},
   keywords = {Voltage-Sensitive Dye; Motion Artifact; Ischemia; Calcium},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological Research},
   title = {Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart},
   url = {http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=2&SID=P2OFFFD6GA56C3HOpNO&page=1&doc=1},
   volume = {vol. 59},
   year = {2010}
}

TY  - JOUR
ID  - 895673
AU  - Kolářová, Jana - Fialová, Kateřina - Janoušek, Oto - Nováková, Marie - Provazník, Ivo
PY  - 2010
TI  - Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart
JF  - Physiological Research
VL  - vol. 59
IS  - Suppl 1
SP  - S71-S80
EP  - S71-S80
SN  - 08628408
KW  - Voltage-Sensitive Dye
KW  - Motion Artifact
KW  - Ischemia
KW  - Calcium
UR  - http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=2&SID=P2OFFFD6GA56C3HOpNO&page=1&doc=1
N2  - Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.
ER  -

KOLÁŘOVÁ, Jana, Kateřina FIALOVÁ, Oto JANOUŠEK, Marie NOVÁKOVÁ a Ivo PROVAZNÍK. Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart. \textit{Physiological Research}. 2010, vol. 59, Suppl 1, s.~S71-S80, 10 s. ISSN~0862-8408.

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