| KOLÁŘOVÁ, Jana, Oto JANOUŠEK, Marie NOVÁKOVÁ, Kateřina FIALOVÁ a Ivo PROVAZNÍK. Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method. Computers in Cardiology. USA, 2009, roč. 36, č. 1, s. 525-528. ISSN 0276-6574. |
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@article{895851,
author = {Kolářová, Jana and Janoušek, Oto and Nováková, Marie and Fialová, Kateřina and Provazník, Ivo},
article_location = {USA},
article_number = {1},
keywords = {voltage sensitive dye; monophasic action potential; isolated heart; ischemia; reperfusion},
language = {eng},
issn = {0276-6574},
journal = {Computers in Cardiology},
note = {36th Annual Conference of Computers in Cardiology},
title = {Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method},
volume = {36},
year = {2009}
}
TY - JOUR
ID - 895851
AU - Kolářová, Jana - Janoušek, Oto - Nováková, Marie - Fialová, Kateřina - Provazník, Ivo
PY - 2009
TI - Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method
JF - Computers in Cardiology
VL - 36
IS - 1
SP - 525-528
EP - 525-528
SN - 02766574
N1 - 36th Annual Conference of Computers in Cardiology
KW - voltage sensitive dye
KW - monophasic action potential
KW - isolated heart
KW - ischemia
KW - reperfusion
N2 - The influence of ischemia and reperfusion duration on left ventricular depolarization was studied from electrograms (ECG) and monophasic action potentials (MAPs). Three orthogonal ECG signals and MAPs were recorded simultaneously during ischemia in isolated rabbit hearts perfused according to Langendorff. MAPs were recorded by optical method via fluorescence measurement from the heart surface at appointed place. The principle of optical measurement of MAP is an application of voltage-sensitive dye (VSD), di-4-ANEPPS in our experimental set-up, into the heart tissue. Optical signal therefore depends on cardiac action potential. The ratiometric fluorescence method, based on emitted light detection by a segment narrow-band photodiode at two different wavelengths, was used. MAPs were recorded with high signal-to-noise ratio and at 2kHz sampling frequency. The speed of depolarization propagation in ventricular tissue was derived from activation time (AT).
ER -
KOLÁŘOVÁ, Jana, Oto JANOUŠEK, Marie NOVÁKOVÁ, Kateřina FIALOVÁ a Ivo PROVAZNÍK. Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method. \textit{Computers in Cardiology}. USA, 2009, roč.~36, č.~1, s.~525-528. ISSN~0276-6574.
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