2010
Signalling mechanisms involved in the inhibition of gap-junctional intercellular communication by cyanobacterial extracts
ADAMOVSKÝ, Ondřej, Luděk BLÁHA, Pavel BABICA, Klára HILSCHEROVÁ, Brad UPHAM et. al.Základní údaje
Originální název
Signalling mechanisms involved in the inhibition of gap-junctional intercellular communication by cyanobacterial extracts
Název česky
Signalní mechanismy ovlivněné inhibicí mezibuňečné komunikace sinicovými toxiny
Autoři
ADAMOVSKÝ, Ondřej (203 Česká republika, garant), Luděk BLÁHA (203 Česká republika), Pavel BABICA (203 Česká republika), Klára HILSCHEROVÁ (203 Česká republika) a Brad UPHAM (840 Spojené státy)
Vydání
The 8th international conference on toxic cyanobacteria, Istanbul, Turkey, 2010
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30304 Public and environmental health
Stát vydavatele
Turecko
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/10:00048814
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
sinocové toxiny promoce karcinogeneze
Klíčová slova anglicky
cyanobacterial toxins tumor promotion
Změněno: 6. 9. 2010 14:12, Mgr. Ondřej Adamovský, Ph.D.
V originále
We investigated effects of various cyanobacterial extracts and pure cyanotoxin microcystin-LR on the inhibition of gap-junctional intercellular communication (GJIC) along with activations of mitogen-activated protein kinases (MAPKs) in normal rat liver stem-like WB-F344 cell line. Modulation of both parameters is an important in vitro biomarker of tumor promotional potencies. Pure microcystin did not inhibit GJIC or activate MAPKs, but complex extracts of water blooms significantly inhibited GJIC and activated MAPK, independent from the content of microcystin. The most pronounced effects were systematically observed in extracts of the cultures of Aphanizomenon flos-aquae as well as in complex water bloom extract dominated by the same species, which does not produce microcystin. Microcystis sp. culture or water bloom had significant but less pronounced effects on both GJIC inhibition and MAPK activation. We have also investigated possible mechanisms controlling the inhibition of GJIC by cyanobacterial extract by using inhibitors of different signaling pathways such as PC-PLC, ERK, PKA and others.
Česky
We investigated effects of various cyanobacterial extracts and pure cyanotoxin microcystin-LR on the inhibition of gap-junctional intercellular communication (GJIC) along with activations of mitogen-activated protein kinases (MAPKs) in normal rat liver stem-like WB-F344 cell line. Modulation of both parameters is an important in vitro biomarker of tumor promotional potencies. Pure microcystin did not inhibit GJIC or activate MAPKs, but complex extracts of water blooms significantly inhibited GJIC and activated MAPK, independent from the content of microcystin. The most pronounced effects were systematically observed in extracts of the cultures of Aphanizomenon flos-aquae as well as in complex water bloom extract dominated by the same species, which does not produce microcystin. Microcystis sp. culture or water bloom had significant but less pronounced effects on both GJIC inhibition and MAPK activation. We have also investigated possible mechanisms controlling the inhibition of GJIC by cyanobacterial extract by using inhibitors of different signaling pathways such as PC-PLC, ERK, PKA and others.
Návaznosti
| GA524/08/0496, projekt VaV |
|