| ZEISBERGEROVÁ, Marta, Roman ŘEMÍNEK, Aleš MÁDR, Zdeněk GLATZ, Jos HOOGMARTENS a Ann VAN SCHEPDAEL. On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection. Electrophoresis. Weinheim: WILEY-VCH Verlag GmbH, 2010, roč. 31, č. 19, s. 3256-3262. ISSN 0173-0835. |
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@article{898963,
author = {Zeisbergerová, Marta and Řemínek, Roman and Mádr, Aleš and Glatz, Zdeněk and Hoogmartens, Jos and Van Schepdael, Ann},
article_location = {Weinheim},
article_number = {19},
keywords = {CE; Cytochrome P450; Dextromethorphan; Metabolism;},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection},
volume = {31},
year = {2010}
}
TY - JOUR
ID - 898963
AU - Zeisbergerová, Marta - Řemínek, Roman - Mádr, Aleš - Glatz, Zdeněk - Hoogmartens, Jos - Van Schepdael, Ann
PY - 2010
TI - On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection
JF - Electrophoresis
VL - 31
IS - 19
SP - 3256-3262
EP - 3256-3262
PB - WILEY-VCH Verlag GmbH
SN - 01730835
KW - CE
KW - Cytochrome P450
KW - Dextromethorphan
KW - Metabolism;
N2 - This study presents the in-capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptors antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused-silica capillary using 100mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2-propanol (10%, v/v). This BGE was suitable for monitoring both off-line and in-capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20mM sodium phosphate, pH 7.4). Finally, in-capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes.
ER -
ZEISBERGEROVÁ, Marta, Roman ŘEMÍNEK, Aleš MÁDR, Zdeněk GLATZ, Jos HOOGMARTENS a Ann VAN SCHEPDAEL. On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection. \textit{Electrophoresis}. Weinheim: WILEY-VCH Verlag GmbH, 2010, roč.~31, č.~19, s.~3256-3262. ISSN~0173-0835.
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