On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection

ZEISBERGEROVÁ, Marta, Roman ŘEMÍNEK, Aleš MÁDR, Zdeněk GLATZ, Jos HOOGMARTENS and Ann VAN SCHEPDAEL. On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection. Electrophoresis. Weinheim: WILEY-VCH Verlag GmbH, 2010, vol. 31, No 19, p. 3256-3262. ISSN 0173-0835.

Basic information

Original name

On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection

Name in Czech

On-line studie metabolismu léčiv pomocí CE

Authors

ZEISBERGEROVÁ, Marta (203 Czech Republic, guarantor), Roman ŘEMÍNEK (203 Czech Republic), Aleš MÁDR (203 Czech Republic), Zdeněk GLATZ (203 Czech Republic), Jos HOOGMARTENS (56 Belgium) and Ann VAN SCHEPDAEL (56 Belgium)

Edition

Electrophoresis, Weinheim, WILEY-VCH Verlag GmbH, 2010, 0173-0835

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.569

RIV identification code

RIV/00216224:14310/10:00044833

Organization unit

Faculty of Science

UT WoS

000283639300010

Keywords (in Czech)

CE; Cytochrom P450; Dextromethorphan; Metabolismus léčiv;

Keywords in English

CE; Cytochrome P450; Dextromethorphan; Metabolism;

Tags

International impact, Reviewed

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Abstract

V originále

This study presents the in-capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptors antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused-silica capillary using 100mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2-propanol (10%, v/v). This BGE was suitable for monitoring both off-line and in-capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20mM sodium phosphate, pH 7.4). Finally, in-capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes.

In Czech

Bla provedena on-line studie metabolismu léčiv pomocí CE

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Links

GAP206/10/0057, research and development project	Name: Miniaturizovaný systém pro "on-line" studie metabolismu léčiv založený na kapilární elektroforéze Investor: Czech Science Foundation, Miniaturized on-line drug metabolism system based on capillary electrophoresis
LC06023, research and development project	Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622413, plan (intention)	Name: Proteiny v metabolismu a při interakci organismů s prostředím Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment