Optimization of immunomagnetic selection of myeloma cells from bone marrow using magnetic activated cell sorting

ČUMOVÁ, Jana, Lucie KOVÁŘOVÁ, Anna POTÁČOVÁ, Ivana BUREŠOVÁ, Fedor KRYUKOV, Miroslav PENKA, Jaroslav MICHÁLEK and Roman HÁJEK. Optimization of immunomagnetic selection of myeloma cells from bone marrow using magnetic activated cell sorting. International journal of hematology. USA: Springer, 2010, vol. 92, No 2, p. 314-319. ISSN 0925-5710.

Basic information

• Original name: Optimization of immunomagnetic selection of myeloma cells from bone marrow using magnetic activated cell sorting
• Name in Czech: Optimalizace imunomagnetické separace myelomových buněk z kostní dřeně
• Authors: ČUMOVÁ, Jana (203 Czech Republic, belonging to the institution), Lucie KOVÁŘOVÁ (203 Czech Republic, belonging to the institution), Anna POTÁČOVÁ (203 Czech Republic, belonging to the institution), Ivana BUREŠOVÁ (203 Czech Republic, belonging to the institution), Fedor KRYUKOV (643 Russian Federation, belonging to the institution), Miroslav PENKA (203 Czech Republic, belonging to the institution), Jaroslav MICHÁLEK (203 Czech Republic, belonging to the institution) and Roman HÁJEK (203 Czech Republic, guarantor, belonging to the institution).
• Edition: International journal of hematology, USA, Springer, 2010, 0925-5710.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.324
• RIV identification code: RIV/00216224:14110/10:00040795
• Organization unit: Faculty of Medicine
• UT WoS: 000281694700016
• Keywords (in Czech): mnohočetný myelom; imunomagnetická separace; plasmatická buňka; CD138
• Keywords in English: multiple myeloma; immunomagnetic separation; plasma cell; CD138

Abstract

Plasma cells (PCs) enrichment from bone marrow samples of multiple myeloma (MM) patients is frequently performed by immunomagnetic separation (magnetic activated cell sorting, MACS) using anti-CD138 MicroBeads. The aim of our work was to find optimal strategy for immunomagnetic separation of PCs and determine optimal algorithm of separation techniques for samples with various percentage of neoplastic cells.

Abstract (in Czech)

Publikace shrnuje naše zkušenosti se separací myelomových buněk z kostní dřeně a možnosti využití těchto vzorků při dalších analýzách. Cílem práce bylo nastavit optimální separační strategii pro vzorky kostní dřeně s různou infiltrací plazmatickými buňkami.

Links

• GAP304/10/1395, research and development project: Name: Analýza klonálních progenitorů plazmatických buněk u monoklonálních gamapatií

Investor: Czech Science Foundation

• LC06027, research and development project: Name: Univerzitní výzkumné centrum - Česká myelomová skupina (Acronym: LC MGUS)

Investor: Ministry of Education, Youth and Sports of the CR, University Research Centre - Czech Myeloma Group

• MSM0021622434, plan (intention): Name: Od klasických prognostických markerů ke klinicky aplikovatelným farmakogenomickým a farmakoproteomickým projektům u mnohočetného myelomu a monoklonálních gamapatií

Investor: Ministry of Education, Youth and Sports of the CR, From classic prognostic markers to clinical applications in selected pharmacogenomic and pharmacoproteomic projects in multiple myeloma and monoclonal gammapathies

• NS10207, research and development project: Name: Úloha abnormalit chromozómu 1 a kaskády NF-kappaB v patogenezi mnohočetného myelomu

Investor: Ministry of Health of the CR