Comparison of Voltage-Sensitive Dye di-4-ANEPPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit

FIALOVÁ, Kateřina, Jana KOLÁŘOVÁ, Ivo PROVAZNÍK and Marie NOVÁKOVÁ. Comparison of Voltage-Sensitive Dye di-4-ANEPPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit. In Computing in Cardiology. 2010. ISSN 0276-6574.

Basic information

• Original name: Comparison of Voltage-Sensitive Dye di-4-ANEPPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit
• Name in Czech: Srovnání účinků napěťově citlivého barviva di-4-ANEPPS u modelu izolovaného srdce potkana, morčete a králíka
• Authors: FIALOVÁ, Kateřina, Jana KOLÁŘOVÁ, Ivo PROVAZNÍK and Marie NOVÁKOVÁ.
• Edition: Computing in Cardiology, 2010.

Other information

• Type of outcome: Conference abstract
• Field of Study: 30105 Physiology
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Organization unit: Faculty of Medicine
• ISSN: 0276-6574
• Keywords in English: voltage-sensitive dye;isolated heart; rat; guinea pig; rabbit; arrhythmia
• Tags: International impact, Reviewed

Abstract

Voltage-sensitive dyes (VSDs) are used for recording of monophasic action potentials (MAPs) by optical method in excitable tissues. Their direct effects on the tissue are not completely elucidated. Previously we studied effects of VSD di-4-ANEPPS during staining and washout in Langendorff-perfused guinea pig and rabbit isolated hearts. However, most often used species in basic cardiology is rat. Therefore, we decided to compare the electrophysiological effects of VSD in isolated hearts of rabbit, guinea pig, and rat. Touch-less electrogram was recorded, the heart rate assessed and normalized. The type and incidence of arrhythmias were evaluated. Based on our results - although the procedure of staining with the dye affects electrophysiological properties of the myocardium in all studied species - rat heart may be considered as the most suitable for electrophysiological studies using di-4-ANEPPS.

Links

• GA102/07/1473, research and development project: Name: Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací

Investor: Czech Science Foundation

• MSM0021622402, plan (intention): Name: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases