2011
Image Division Technique in Pre-acquisition Analysis of Information Content for Automated Microscopy
BRÁZDILOVÁ, Silvie Luisa a Michal KOZUBEKZákladní údaje
Originální název
Image Division Technique in Pre-acquisition Analysis of Information Content for Automated Microscopy
Autoři
BRÁZDILOVÁ, Silvie Luisa (203 Česká republika, domácí) a Michal KOZUBEK (203 Česká republika, garant, domácí)
Vydání
Journal of Microscopy, Oxford, Blackwell Science, 2011, 0022-2720
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.631
Kód RIV
RIV/00216224:14330/11:00051479
Organizační jednotka
Fakulta informatiky
UT WoS
000290627600006
Klíčová slova anglicky
Automated microscopy; fluorescence microscopy; autofocusing; multimodal content function; image acquisition; information content analysis
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 10. 3. 2018 16:44, prof. RNDr. Michal Kozubek, Ph.D.
Anotace
V originále
This article presents a method that allows for reliable automated image acquisition of specimens with high information content in light microscopy with emphasis on fluorescence microscopy applications. Automated microscopy typically relies on autofocusing used for the analysis of information content behaviour along z-axis within each field of view. However, in the case of a field of view containing more objects that do not lie precisely in one z-plane, traditional autofocusing methods fail due to their principle of operation. We avoid this issue by reducing the original problem to a set of simple and performable tasks: we divide the field of view into a small number of tiles and process each of them individually. The obtained results enable discovering z-planes with rich information content that remain hidden during global analysis of the whole field of view. Our approach therefore outperforms other acquisition methods including the manual one. A large part of the contribution is oriented toward practical application.
Návaznosti
LC535, projekt VaV |
| ||
MSM0021622419, záměr |
| ||
MUNI/A/0914/2009, interní kód MU |
| ||
2B06052, projekt VaV |
|