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@proceedings{902373, author = {Bouchal, Pavel and Mudrochová, Monika and Struhárová, Iva and Roumeliotis, Theodoros and Hernychová, Lenka and Hrstka, Roman and Garbis, Spiros D. and Nenutil, Rudolf and Vojtěšek, Bořivoj}, keywords = {breast cancer; metastasis; biomarkers; proteomics}, language = {eng}, title = {Biomarkers of metastatic potential in low grade breast cancer}, url = {http://www.selectbiosciences.com/conferences/cp2010/}, year = {2010} }
TY - CONF ID - 902373 AU - Bouchal, Pavel - Mudrochová, Monika - Struhárová, Iva - Roumeliotis, Theodoros - Hernychová, Lenka - Hrstka, Roman - Garbis, Spiros D. - Nenutil, Rudolf - Vojtěšek, Bořivoj PY - 2010 TI - Biomarkers of metastatic potential in low grade breast cancer KW - breast cancer KW - metastasis KW - biomarkers KW - proteomics UR - http://www.selectbiosciences.com/conferences/cp2010/ N2 - Our effort is focused on discovery, evaluation and validation of perspective protein markers of metastatic potential in low grade breast cancer biopsies. Three proteomics approaches have been involved in complex protein composition analysis of clinically and pathologically well characterized set of tissues: SELDI-TOF MS, 2-D PAGE and iTRAQ-2DLC-MS/MS. This combined approach enabled us to simultaneously identify and quantify more than 600 proteins using LC-MS/MS and to detect more than 800 protein spots using 2-D PAGE followed by MS/MS protein identification. The results involving differential levels of several biologically relevant groups of proteins are discussed on the poster. ER -
BOUCHAL, Pavel, Monika MUDROCHOVÁ, Iva STRUHÁROVÁ, Theodoros ROUMELIOTIS, Lenka HERNYCHOVÁ, Roman HRSTKA, Spiros D. GARBIS, Rudolf NENUTIL a Bořivoj VOJTĚŠEK. \textit{Biomarkers of metastatic potential in low grade breast cancer}. 2010.
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