| DANNAOUI, Eric, Patric SCHWARZ, Michal SLANÝ, Juergen LOEFFLER, Anne Tomine JORDE, Manuel CUENCA-ESTRELLA, Philipe M HAUSER, Raghdaa SHRIEF, M. HUERRE, Tomáš FREIBERGER, Peter GAUSTAD, Juan-Luis RODRIGUEZ-TUDELA, Jaques BILLE, David W. DENNING, Stéphane BRETAGNE a Olivier LORTHOLARY. Molecular Detection and Identification of Zygomycetes Species from Paraffin-Embedded Tissues in a Murine Model of Disseminated Zygomycosis: a Collaborative European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Fungal Infection Study. Journal of Clinical Microbiology. J. Clin. Microbiol., 2010, roč. 2010, č. 48, s. 2043-2046. ISSN 0095-1137. |
Další formáty:
BibTeX
LaTeX
RIS
@article{903060,
author = {Dannaoui, Eric and Schwarz, Patric and Slaný, Michal and Loeffler, Juergen and Jorde, Anne Tomine and CuencaandEstrella, Manuel and Hauser, Philipe M and Shrief, Raghdaa and Huerre, M. and Freiberger, Tomáš and Gaustad, Peter and RodriguezandTudela, JuanandLuis and Bille, Jaques and Denning, David W. and Bretagne, Stéphane and Lortholary, Olivier},
article_number = {48},
keywords = {zygomycetes; PCR},
language = {eng},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
title = {Molecular Detection and Identification of Zygomycetes Species from Paraffin-Embedded Tissues in a Murine Model of Disseminated Zygomycosis: a Collaborative European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Fungal Infection Study},
volume = {2010},
year = {2010}
}
TY - JOUR
ID - 903060
AU - Dannaoui, Eric - Schwarz, Patric - Slaný, Michal - Loeffler, Juergen - Jorde, Anne Tomine - Cuenca-Estrella, Manuel - Hauser, Philipe M - Shrief, Raghdaa - Huerre, M. - Freiberger, Tomáš - Gaustad, Peter - Rodriguez-Tudela, Juan-Luis - Bille, Jaques - Denning, David W. - Bretagne, Stéphane - Lortholary, Olivier
PY - 2010
TI - Molecular Detection and Identification of Zygomycetes Species from Paraffin-Embedded Tissues in a Murine Model of Disseminated Zygomycosis: a Collaborative European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Fungal Infection Study
JF - Journal of Clinical Microbiology
VL - 2010
IS - 48
SP - 2043-2046
EP - 2043-2046
PB - J. Clin. Microbiol.
SN - 00951137
KW - zygomycetes
KW - PCR
N2 - The present study was performed to assess the interlaboratory reproducibility of the molecular detection and identification of species of Zygomycetes from formalin-fixed paraffin-embedded kidney and brain tissues obtained from experimentally infected mice. Animals were infected with one of five species (Rhizopus oryzae, Rhizopus microsporus, Lichtheimia corymbifera, Rhizomucor pusillus, and Mucor circinelloides). Samples with 1, 10, or 30 slide cuts of the tissues were prepared from each paraffin block, the sample identities were blinded for analysis, and the samples were mailed to each of seven laboratories for the assessment of sensitivity. With the protocol used in the present study, the interlaboratory reproducibility of ITS sequencing for the identification of major Zygomycetes species from formalin-fixed paraffin-embedded tissues can reach 100%, when enough material is available.
ER -
DANNAOUI, Eric, Patric SCHWARZ, Michal SLANÝ, Juergen LOEFFLER, Anne Tomine JORDE, Manuel CUENCA-ESTRELLA, Philipe M HAUSER, Raghdaa SHRIEF, M. HUERRE, Tomáš FREIBERGER, Peter GAUSTAD, Juan-Luis RODRIGUEZ-TUDELA, Jaques BILLE, David W. DENNING, Stéphane BRETAGNE a Olivier LORTHOLARY. Molecular Detection and Identification of Zygomycetes Species from Paraffin-Embedded Tissues in a Murine Model of Disseminated Zygomycosis: a Collaborative European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Fungal Infection Study. \textit{Journal of Clinical Microbiology}. J. Clin. Microbiol., 2010, roč.~2010, č.~48, s.~2043-2046. ISSN~0095-1137.
|
