In vitro activation of CMV-specific human CD8+ T cells by CyaA
toxoids delivering pp65 epitopes

a 2010

In vitro activation of CMV-specific human CD8+ T cells by CyaA toxoids delivering pp65 epitopes

JELÍNEK, Jiří, Irena ADKINS, P. ŠEBO a Jaroslav MICHÁLEK

Základní údaje

Originální název

In vitro activation of CMV-specific human CD8+ T cells by CyaA toxoids delivering pp65 epitopes

Autoři

JELÍNEK, Jiří, Irena ADKINS, P. ŠEBO a Jaroslav MICHÁLEK

Vydání

1st International Conference on Advances in Cell and Gene Therapy and Immunotherapy: from basic research to clinical applications and 3rd Workshop on Immunotherapy. 2010

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

ISBN

978-80-7392-137-8

Klíčová slova anglicky

T cells; CyaA toxoid; immunotherapy

Změněno: 25. 10. 2010 07:54, Mgr. Anna Potáčová, Ph.D.

Anotace

V originále

In this study, we investigated the capacity of two CyaA constructs to deliver epitopes from the CMV phosphoprotein pp65 to activate T cells of CMV-seropositive and CMV-seronegative healthy donors in vitro. Immune response of T cells was monitored using Flow cytometry and Cytometric Beads Array. Expansion of CMV-specific T cells secreting gamma-interferon as well as increase of total IFN-gamma and TNF-alfa; production could be detected after in vitro stimulation with CyaA-CMV constructs, while limited activation of T cells occurred with free peptides. No immune response was evoked by control CyaA without CMV antigenic peptides. These data reveal the potential of CyaA constructs as a tool for fast and reliable detection of CMV-specific immunity and for immunoprophylactic, as well as immunotherapeutic application against CMV-associated diseases in immunocompromised patients.

Návaznosti

NS9871, projekt VaV

Název: Využití dendritických buněk naložených univerzálními nádorovými antigeny pro přípravu protinádorových vakcín

2B06058, projekt VaV

Název: Bezpečná biotechnologie alotransplantovaných hematopoetických kmenových buněk u vybraných solidních a hematologických nádorů jako adoptivní imunoterapie (Akronym: NPVII11001)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Bezpečná biotechnologie alotransplatovaných hematopoetických kmenových buněk u vybraných solidních a hematologických nádorů jako adoptivní imunoterapie

Citovat

JELÍNEK, Jiří, Irena ADKINS, P. ŠEBO a Jaroslav MICHÁLEK. In vitro activation of CMV-specific human CD8+ T cells by CyaA toxoids delivering pp65 epitopes. In 1st International Conference on Advances in Cell and Gene Therapy and Immunotherapy: from basic research to clinical applications and 3rd Workshop on Immunotherapy. 2010. ISBN 978-80-7392-137-8.

@proceedings{904164,
   author = {Jelínek, Jiří and Adkins, Irena and Šebo, P. and Michálek, Jaroslav},
   booktitle = {1st International Conference on Advances in Cell and Gene Therapy and Immunotherapy: from basic research to clinical applications and 3rd Workshop on Immunotherapy.},
   keywords = {T cells; CyaA toxoid; immunotherapy},
   language = {eng},
   isbn = {978-80-7392-137-8},
   title = {In vitro activation of CMV-specific human CD8+ T cells by CyaA toxoids delivering pp65 epitopes},
   year = {2010}
}

TY  - CONF
ID  - 904164
AU  - Jelínek, Jiří - Adkins, Irena - Šebo, P. - Michálek, Jaroslav
PY  - 2010
TI  - In vitro activation of CMV-specific human CD8+ T cells by CyaA toxoids delivering pp65 epitopes
SN  - 9788073921378
KW  - T cells
KW  - CyaA toxoid
KW  - immunotherapy
N2  - In this study, we investigated the capacity of two CyaA constructs to deliver epitopes from the CMV phosphoprotein pp65 to activate T cells of CMV-seropositive and CMV-seronegative healthy donors in vitro. Immune response of T cells was monitored using Flow cytometry and Cytometric Beads Array. Expansion of CMV-specific T cells secreting gamma-interferon as well as increase of total IFN-gamma and TNF-alfa; production could be detected after in vitro stimulation with CyaA-CMV constructs, while limited activation of T cells occurred with free peptides. No immune response was evoked by control CyaA without CMV antigenic peptides. These data reveal the potential of CyaA constructs as a tool for fast and reliable detection of CMV-specific immunity and for immunoprophylactic, as well as immunotherapeutic application against CMV-associated diseases in immunocompromised patients.
ER  -

JELÍNEK, Jiří, Irena ADKINS, P. ŠEBO a Jaroslav MICHÁLEK. In vitro activation of CMV-specific human CD8+ T cells by CyaA toxoids delivering pp65 epitopes. In \textit{1st International Conference on Advances in Cell and Gene Therapy and Immunotherapy: from basic research to clinical applications and 3rd Workshop on Immunotherapy.}. 2010. ISBN~978-80-7392-137-8.

Podrobný výpis o publikaci