| STEJSKAL, Stanislav, Pavel MATULA, Ondřej DANĚK, Martin MAŠKA, Irena KRONTORÁD KOUTNÁ a Michal KOZUBEK. A novel approach to evaluation of the spatial relationship of fluorescent signals in ImunnoFISH analysis of different kind. In Advances in Molecular and Cancer Biology, 2010. Brno: muni PRESS, 2010, s. 39-41. ISBN 978-80-210-5312-0. |
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@inproceedings{907859,
author = {Stejskal, Stanislav and Matula, Pavel and Daněk, Ondřej and Maška, Martin and Krontorád Koutná, Irena and Kozubek, Michal},
address = {Brno},
booktitle = {Advances in Molecular and Cancer Biology, 2010},
keywords = {confocal microscopy; image analysis; fluorescent in situ hybridisation; immunoflourescence; nucleus},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Brno},
isbn = {978-80-210-5312-0},
pages = {39-41},
publisher = {muni PRESS},
title = {A novel approach to evaluation of the spatial relationship of fluorescent signals in ImunnoFISH analysis of different kind.},
year = {2010}
}
TY - JOUR
ID - 907859
AU - Stejskal, Stanislav - Matula, Pavel - Daněk, Ondřej - Maška, Martin - Krontorád Koutná, Irena - Kozubek, Michal
PY - 2010
TI - A novel approach to evaluation of the spatial relationship of fluorescent signals in ImunnoFISH analysis of different kind.
PB - muni PRESS
CY - Brno
SN - 9788021053120
KW - confocal microscopy
KW - image analysis
KW - fluorescent in situ hybridisation
KW - immunoflourescence
KW - nucleus
N2 - Confocal fluorescence microscopy enables the study and observation of the different structures in the cell in the high resolution. Its role in epigenetic research is finding spatial distribution and interaction of factors that can affect gene expression without change in genetic code. Combination of specific staining of DNA sequences and nuclear proteins (ImmunoFISH) help us to describe the protein distribution in the neighbourhood of targeted genes. In our early studies we had focused on the mutual spatial relation between genetic loci with described genetic activity and nuclear proteins commonly linked with the regulation of gene activity. Unfortunately, a broad distribution of the protein signal disabled the classical co-localisation analysis and we had to find an alternative approach for image analysis. Output of the presented image analysis is helpful for the co-localisation analysis of image data with broad distribution of signal and suitable for statistical analysis. All steps of analysis were implemented into the Acquiarium software.
ER -
STEJSKAL, Stanislav, Pavel MATULA, Ondřej DANĚK, Martin MAŠKA, Irena KRONTORÁD KOUTNÁ a Michal KOZUBEK. A novel approach to evaluation of the spatial relationship of fluorescent signals in ImunnoFISH analysis of different kind. In \textit{Advances in Molecular and Cancer Biology, 2010}. Brno: muni PRESS, 2010, s.~39-41. ISBN~978-80-210-5312-0.
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