2011
Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood
KRONTORÁD KOUTNÁ, Irena, Martina PETERKOVÁ, Pavel ŠIMARA, Stanislav STEJSKAL, Lenka TESAŘOVÁ et. al.Základní údaje
Originální název
Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood
Název česky
Proliferační a diferenciační potenciál CD133+ a CD34+ buněčných populací kostní dřeně a periferní mobilizované krve
Autoři
KRONTORÁD KOUTNÁ, Irena (203 Česká republika, garant, domácí), Martina PETERKOVÁ (203 Česká republika, domácí), Pavel ŠIMARA (203 Česká republika, domácí), Stanislav STEJSKAL (203 Česká republika, domácí), Lenka TESAŘOVÁ (203 Česká republika, domácí) a Michal KOZUBEK (203 Česká republika, domácí)
Vydání
Annals of hematology, Germany, Springer, 2011, 0939-5555
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30200 3.2 Clinical medicine
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.615
Kód RIV
RIV/00216224:14330/11:00051523
Organizační jednotka
Fakulta informatiky
UT WoS
000286053500001
Klíčová slova česky
CD34 ;CD133; HSC; transplantace; VEGFR
Klíčová slova anglicky
CD34; CD133; HSC; transplantation; VEGFR
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 13. 4. 2011 12:53, doc. RNDr. Irena Koutná, Ph.D.
Anotace
V originále
CD34 is the most frequently used marker for the selection of cells for bone marrow (BM) transplantation. The use of CD133 as an alternative marker is an open research topic. The goal of this study was to evaluate the proliferation and differentiation potential for hematopoiesis (short and long term) of CD133+ and CD34+ populations from bone marrow and mobilized peripheral blood. Eight cell populations were compared: CD34+ and CD133+ cells from both the BM (CML Ph-, CML Ph+, and healthy volunteers) and mobilized peripheral blood cells. Multicolor flow cytometry and cultivation experiments were used to measure expression and differentiation of the individual populations. It was observed that the CD133+ BM population showed higher cell expansion. Another finding is that during a 6-day cultivation with 5(6)-carboxyfluorescein diacetate N- succinimidyl ester (CFSE), more cells remained in division D0 (non-dividing cells). There was a higher percentage of CD38- cells observed on the CD133+ BM population. It was also observed that the studied populations contained very similar but not the same pools of progenitors: erythroid, lymphoid, and myeloid. This was confirmed by CFU-GM and CFU-E experiments. The VEGFR antigen was used to monitor subpopulations of endothelial sinusoidal progenitors. The CD133+ BM population contained significantly more VEGFR+ cells. Our findings suggest that the CD133+ population from the BM shows better proliferation activity and a higher distribution of primitive progenitors than any other studied population.
Návaznosti
| MSM0021622430, záměr |
| ||
| 2B06052, projekt VaV |
|