| ŽERAVÍK, Jiří, Karel LACINA, Milan JÍLEK, Jiří VLČEK and Petr SKLÁDAL. Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase. Microchimica Acta. Wien (Austria): Springer, 2010, vol. 170, 3-4, p. 251-256. ISSN 0026-3672. |
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@article{908995,
author = {Žeravík, Jiří and Lacina, Karel and Jílek, Milan and Vlček, Jiří and Skládal, Petr},
article_location = {Wien (Austria)},
article_number = {3-4},
keywords = {Biosensor; Sarcosine oxidase; Carboxylic acid; Wine;},
language = {eng},
issn = {0026-3672},
journal = {Microchimica Acta},
title = {Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase},
volume = {170},
year = {2010}
}
TY - JOUR
ID - 908995
AU - Žeravík, Jiří - Lacina, Karel - Jílek, Milan - Vlček, Jiří - Skládal, Petr
PY - 2010
TI - Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase
JF - Microchimica Acta
VL - 170
IS - 3-4
SP - 251-256
EP - 251-256
PB - Springer
SN - 00263672
KW - Biosensor
KW - Sarcosine oxidase
KW - Carboxylic acid
KW - Wine;
N2 - The flow-through amperometric biosensor is presented for determination of carboxylic acids. It is based on two sensor layers that are deposited on a platinum electrode. The inner layer serves to eliminate interferences by limiting diffusion of electrochemically active substances such as ascorbic acid. This layer is electro-polymerized using an equimolar mixture of o-phenylenediamine and resorcinol. The outer layer is prepared by cross-linking the enzyme sarcosine oxidase and bovine serum albumin using glutaraldehyde. The formation of enzymatically produced hydrogen peroxide is monitored at 600 mV vs. an Ag/AgCl reference electrode. The addition of carboxylic acids causes competitive inhibition of the enzyme and a decrease in signal. The assay was optimized for determination of carboxylic acids in wine samples. Following 10-fold dilution, most samples contain 1-10 mM individual carboxylic acids and thus a 5 mM concentration of sarcosine was chosen as being optimal for competition.
ER -
ŽERAVÍK, Jiří, Karel LACINA, Milan JÍLEK, Jiří VLČEK and Petr SKLÁDAL. Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase. \textit{Microchimica Acta}. Wien (Austria): Springer, 2010, vol.~170, 3-4, p.~251-256. ISSN~0026-3672.
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