125I-labelled iodothyronines: useful tools for studies of effects of an antidepressant drug fluoxetine in the rat

PAVELKA, Stanislav. 125I-labelled iodothyronines: useful tools for studies of effects of an antidepressant drug fluoxetine in the rat. Journal of Radioanalytical and Nuclear Chemistry. 2010, vol. 286, No 3, p. 867-871. ISSN 0236-5731.

Basic information

• Original name: 125I-labelled iodothyronines: useful tools for studies of effects of an antidepressant drug fluoxetine in the rat
• Name in Czech: 125I-značené jodothyroniny: užitečné nástroje pro studium účinků antidepresiva fluoxetinu u potkana
• Authors: PAVELKA, Stanislav (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Journal of Radioanalytical and Nuclear Chemistry, 2010, 0236-5731.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30104 Pharmacology and pharmacy
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 0.777
• RIV identification code: RIV/00216224:14310/10:00051563
• Organization unit: Faculty of Science
• UT WoS: 000285099700043
• Keywords (in Czech): Antidepresivum; radiometrické enzymové stanovení; tyreoidální hormon
• Keywords in English: Antidepressant drug; radiometric enzyme assay; thyroid hormone
• Tags: AKb, rivok
• Tags: International impact, Reviewed

Abstract

We followed the effects of an antidepressant drug fluoxetine (Fluox), administered sub-chronically to Wistar rats by itself, or in combination with 3,3',5-triiodothyronine. In studies of the interaction of Fluox with the metabolism of thyroid hormones, we applied adapted radiometric enzyme assays for iodothyronine sulfotransferases and uridine 5'-diphospho-glucuronyltransferase, as well as our newly developed radiometric assays for iodothyronine deiodinases of types 1, 2 and 3, using 125I-labelled iodothyronines of high specific radioactivity as substrates.

Abstract (in Czech)

V práci jsme sledovali účinky antidepresiva fluoxetinu (Fluox), subchronicky podávaného potkanům kmene Wistar buď samostatně nebo v kombinaci s 3,3',5-trijodthyroninem. Při studiích interakce Fluox s metabolismem tyreoidálních hormonů jsme použili jednak adaptovaná radiometrická stanovení enzymových aktivit jodothyronin sulfotransferas a uridin 5'-difosfo-glukuronyltransferasy, jednak naše nově vyvinutá radiometrická stanovení enzymových aktivit jodothyronin dejodas typu 1, 2 a 3 s využitím 125I-značených jodothyroninů o vysoké specifické radioaktivitě coby substrátů.

Links

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment