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@proceedings{911265, author = {Žďárská, Markéta and Zatloukalová, Pavlína and Potěšil, David and Šedo, Ondrej and Zdráhal, Zbyněk and Hejátko, Jan}, keywords = {proteome analysis, Arabidopsis, cytokinin}, language = {eng}, title = {Quantitative differential analysis of plant proteome in a response to hormonal regulations}, url = {http://www.geyseco.es/ipgsa2010/index.php?go=welcome}, year = {2010} }
TY - CONF ID - 911265 AU - Žďárská, Markéta - Zatloukalová, Pavlína - Potěšil, David - Šedo, Ondrej - Zdráhal, Zbyněk - Hejátko, Jan PY - 2010 TI - Quantitative differential analysis of plant proteome in a response to hormonal regulations KW - proteome analysis, Arabidopsis, cytokinin UR - http://www.geyseco.es/ipgsa2010/index.php?go=welcome N2 - Cytokinins (CK) have been implicated in regulation of many aspects of plant growth and development. Recent genetic studies suggest that CK play distinct and sometimes even opposite roles in the regulation of shoot and root development in Arabidopsis. However, the underlying molecular mechanisms are still rather elusive. Here we present results of a comprehensive approach in the study of differential shoot and root CK response. To study immediate-early and delayed CK response, six days old seedlings of Arabidopsis th.(Col-0) were treated with 5 uM 6-benzylamino purine for 30 min and 2 hours, respectively. Root and shoot tissue was analysed separately for both studied intervals. The proteome analysis was performed using 2-D gel electrophoresis and luminescent SYPRO Ruby gel staining. Proteins in the selected spots were identified using LC-MS/MS. Applying that approach allowed us to identify a number of unique, qualitatively and/or quantitatively different proteins in the root or shoot samples within both studied intervals. ER -
ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, David POTĚŠIL, Ondrej ŠEDO, Zbyněk ZDRÁHAL and Jan HEJÁTKO. \textit{Quantitative differential analysis of plant proteome in a response to hormonal regulations}. 2010.
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