Identification of Staphylococcus spp. using (GTG)5-PCR
fingerprinting

J 2010

Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting

ŠVEC, Pavel, Roman PANTŮČEK, Petr PETRÁŠ, Ivo SEDLÁČEK, Dana NOVÁKOVÁ et. al.

Základní údaje

Originální název

Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting

Autoři

ŠVEC, Pavel (203 Česká republika, garant, domácí), Roman PANTŮČEK (203 Česká republika, domácí), Petr PETRÁŠ (203 Česká republika), Ivo SEDLÁČEK (203 Česká republika, domácí) a Dana NOVÁKOVÁ (203 Česká republika, domácí)

Vydání

Systematic and Applied Microbiology, Germany, Elsevier GmbH, 2010, 0723-2020

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.075

Kód RIV

RIV/00216224:14310/10:00045696

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000286481200004

Klíčová slova anglicky

Staphylococcus; (GTG)5-PCR; rep-PCR; rpoB gene sequencing; Identification; Taxonomy

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2011 07:26, doc. RNDr. Pavel Švec, Ph.D.

Anotace

V originále

A group of 212 type and reference strains deposited in the Czech Collection of Microorganisms (Brno, Czech Republic) and covering 41 Staphylococcus species comprising 21 subspecies was characterised using rep-PCR fingerprinting with the (GTG)5 primer in order to evaluate this method for identification of staphylococci. All strains were typeable using the (GTG)5 primer and generated PCR products ranging from 200 to 4500 bp. Numerical analysis of the obtained fingerprints revealed (sub)speciesspecific clustering corresponding with the taxonomic position of analysed strains. Taxonomic position of selected strains representing the (sub)species that were distributed over multiple rep-PCR clusters was verified and confirmed by the partial rpoB gene sequencing. Staphylococcus caprae, Staphylococcus equorum, Staphylococcus sciuri, Staphylococcus piscifermentans, Staphylococcus xylosus, and Staphylococcus saprophyticus revealed heterogeneous fingerprints and each (sub)species was distributed over several clusters. However, representatives of the remaining Staphylococcus spp. were clearly separated in single (sub)species-specific clusters. These results showed rep-PCR with the (GTG)5 primer as a fast and reliable method applicable for differentiation and straightforward identification of majority of Staphylococcus spp.

Návaznosti

GA310/09/0459, projekt VaV

Název: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

Investor: Grantová agentura ČR, Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Citovat

ŠVEC, Pavel, Roman PANTŮČEK, Petr PETRÁŠ, Ivo SEDLÁČEK a Dana NOVÁKOVÁ. Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting. Systematic and Applied Microbiology. Germany: Elsevier GmbH, 2010, roč. 33, č. 8, s. 451-456. ISSN 0723-2020.

@article{911367,
   author = {Švec, Pavel and Pantůček, Roman and Petráš, Petr and Sedláček, Ivo and Nováková, Dana},
   article_location = {Germany},
   article_number = {8},
   keywords = {Staphylococcus; (GTG)5-PCR; rep-PCR; rpoB gene sequencing; Identification; Taxonomy},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting},
   url = {http://linkinghub.elsevier.com/retrieve/pii/S0723-2020(10)00139-6},
   volume = {33},
   year = {2010}
}

TY  - JOUR
ID  - 911367
AU  - Švec, Pavel - Pantůček, Roman - Petráš, Petr - Sedláček, Ivo - Nováková, Dana
PY  - 2010
TI  - Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting
JF  - Systematic and Applied Microbiology
VL  - 33
IS  - 8
SP  - 451-456
EP  - 451-456
PB  - Elsevier GmbH
SN  - 07232020
KW  - Staphylococcus
KW  - (GTG)5-PCR
KW  - rep-PCR
KW  - rpoB gene sequencing
KW  - Identification
KW  - Taxonomy
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0723-2020(10)00139-6
N2  - A group of 212 type and reference strains deposited in the Czech Collection of Microorganisms (Brno, Czech Republic) and covering 41 Staphylococcus species comprising 21 subspecies was characterised using rep-PCR fingerprinting with the (GTG)5 primer in order to evaluate this method for identification of staphylococci. All strains were typeable using the (GTG)5 primer and generated PCR products ranging from 200 to 4500 bp. Numerical analysis of the obtained fingerprints revealed (sub)speciesspecific clustering corresponding with the taxonomic position of analysed strains. Taxonomic position of selected strains representing the (sub)species that were distributed over multiple rep-PCR clusters was verified and confirmed by the partial rpoB gene sequencing. Staphylococcus caprae, Staphylococcus equorum, Staphylococcus sciuri, Staphylococcus piscifermentans, Staphylococcus xylosus, and Staphylococcus saprophyticus revealed heterogeneous fingerprints and each (sub)species was distributed over several clusters. However, representatives of the remaining Staphylococcus spp. were clearly separated in single (sub)species-specific clusters. These results showed rep-PCR with the (GTG)5 primer as a fast and reliable method applicable for differentiation and straightforward identification of majority of Staphylococcus spp.
ER  -

ŠVEC, Pavel, Roman PANTŮČEK, Petr PETRÁŠ, Ivo SEDLÁČEK a Dana NOVÁKOVÁ. Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting. \textit{Systematic and Applied Microbiology}. Germany: Elsevier GmbH, 2010, roč.~33, č.~8, s.~451-456. ISSN~0723-2020.

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