2010
Monitoring of Cytochrome P450 2C9 isoform reaction with Diclofenac by In-Capillary System based on Transverse Diffusion of Laminar Flow Profiles
ŘEMÍNEK, Roman, Aleš MÁDR a Zdeněk GLATZZákladní údaje
Originální název
Monitoring of Cytochrome P450 2C9 isoform reaction with Diclofenac by In-Capillary System based on Transverse Diffusion of Laminar Flow Profiles
Název česky
Monitorování reakce CYP2C9 s dikclofenacem v kapiláře
Název anglicky
Monitoring of Cytochrome P450 2C9 isoform reaction with Diclofenac by In-Capillary System based on Transverse Diffusion of Laminar Flow Profiles
Autoři
ŘEMÍNEK, Roman, Aleš MÁDR a Zdeněk GLATZ
Vydání
první. Florianopolis Brazílie, Book of Abtracts 16th Latin-American Symposium LACE 2010, od s. 138-138, 1 s. 2010
Nakladatel
prof. Emanuel Carrilho
Další údaje
Typ výsledku
Stať ve sborníku
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
EMMA; cytochromy P450; metabolismus léčiv; diclofenac
Klíčová slova anglicky
EMMA; cytochromes P450; drug metabolism; diclofenac
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 13. 12. 2010 15:45, prof. RNDr. Zdeněk Glatz, CSc.
V originále
In this study a method for cytochrome P450 2C9 (CYP2C9) reaction with diclofenac as a probe substrate was developed. 30 min incubation time and sandwich layout, where a plug of CYP2C9 is surrounded by two plugs of diclofenac and NADPH mixture, were selected as appropriate incubation conditions for final method within the optimizing. Although bare silica capillary was used for performing the analyses of samples consisting of enzyme preparations, strong rinsing procedure with 1 M H3PO4 and 1 M NaOH applied between following runs provides good method's repeatability with RSD values of 1.52 % and 4.83 % (n = 6) for migration times and peak areas of 4'-hydroxydiclofenac, respectively. Since the methodology of TDLFP allows changing the incubation mixture composition in simple way, the introduced method represents a promising tool for in-capillary inhibition studies of CYP2C9.
Česky
Byla vypracována metoda pro monitorováni reakce CYP2C9 s dikclofenacem v kapiláře
Anglicky
In this study a method for cytochrome P450 2C9 (CYP2C9) reaction with diclofenac as a probe substrate was developed. 30 min incubation time and sandwich layout, where a plug of CYP2C9 is surrounded by two plugs of diclofenac and NADPH mixture, were selected as appropriate incubation conditions for final method within the optimizing. Although bare silica capillary was used for performing the analyses of samples consisting of enzyme preparations, strong rinsing procedure with 1 M H3PO4 and 1 M NaOH applied between following runs provides good method's repeatability with RSD values of 1.52 % and 4.83 % (n = 6) for migration times and peak areas of 4'-hydroxydiclofenac, respectively. Since the methodology of TDLFP allows changing the incubation mixture composition in simple way, the introduced method represents a promising tool for in-capillary inhibition studies of CYP2C9.
Návaznosti
| GAP206/10/0057, projekt VaV |
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| LC06023, projekt VaV |
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| MSM0021622413, záměr |
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