FOJTOVÁ, Miloslava, Petra HORÁKOVÁ, Hana PIVOŇKOVÁ, Zdenka VYCHODILOVÁ and Miroslav FOJTA. Utilization of electrochemical activity of 7-deazaguanine in the monitoring of PCR amplification of G-rich DNA Strands. In XXX. Moderní Elektrochemické Metody. Ústí nad Labem: BEST servis Ústí nad Labem, 2010, 5 pp. ISBN 978-80-254-6710-7.
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Basic information
Original name Utilization of electrochemical activity of 7-deazaguanine in the monitoring of PCR amplification of G-rich DNA Strands.
Authors FOJTOVÁ, Miloslava (203 Czech Republic, guarantor, belonging to the institution), Petra HORÁKOVÁ (203 Czech Republic), Hana PIVOŇKOVÁ (203 Czech Republic, belonging to the institution), Zdenka VYCHODILOVÁ (203 Czech Republic) and Miroslav FOJTA (203 Czech Republic, belonging to the institution).
Edition Ústí nad Labem, XXX. Moderní Elektrochemické Metody, 5 pp. 2010.
Publisher BEST servis Ústí nad Labem
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10610 Biophysics
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/10:00045855
Organization unit Faculty of Science
ISBN 978-80-254-6710-7
UT WoS 000283050900012
Keywords in English OSMIUM-TETROXIDE COMPLEXES; NUCLEOSIDE TRIPHOSPHATES; ETHIDIUM-BROMIDE; HYBRIDIZATION; ASSAY; BASE
Tags Reviewed
Changed by Changed by: prof. RNDr. Jiří Fajkus, CSc., učo 28574. Changed: 20/12/2010 12:16.
Abstract
7-deazaguanine, guanine analogue, is able to be sequence specifically incorporated into DNA. Modified DNA fragments can not be detected by standard techniques (gel electrophoresis, quantitative PCR) because of quenching of fluorescence of commonly used dyes. Electrochemical detection provides 7-deazaguanine-specific signal suitable for the quantification of PCR product and determination of the extent of DNA modification.
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