Neuroinflammatory reaction of DRG following unilateral nerve injury is bilaterally spread alongside neuroaxis

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ and Václav BRÁZDA. Neuroinflammatory reaction of DRG following unilateral nerve injury is bilaterally spread alongside neuroaxis. In 42-nd International Congress on Anatomy and 47-th Lojda Symposium on Histochemistry. 2010. ISBN 978-80-7097-805-4.

Basic information

• Original name: Neuroinflammatory reaction of DRG following unilateral nerve injury is bilaterally spread alongside neuroaxis
• Name in Czech: Neurozánětlivá reakce spinálních ganglií po jednostranném poškození periferního nervu je bilaterálně šířena podél nervové osy
• Authors: DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ and Václav BRÁZDA.
• Edition: 42-nd International Congress on Anatomy and 47-th Lojda Symposium on Histochemistry, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Slovakia
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• ISBN: 978-80-7097-805-4
• Keywords (in Czech): neurozánět, neuropatická bolest, poškození periferního nervu, model neuropatické bolesti
• Keywords in English: neuroinflammation, neuropathic pain, peripheral nerve injury, neuropathic pain model

Abstract

Wallerian degeneration following mechanical injury of the peripheral nerve has a significant inflammatory component (neuroinflammation), primarily due to the production of variety of mediators including cytokines. Compelling evidence indicates that neuropathic pain induced by nerve injury is related to changes in the dorsal root ganglia (DRG) containing bodies of the primary afferent neurons. Unilateral chronic constriction injury (CCI) of the sciatic nerve was performed aseptically under deep anesthesia in sixty fore rats. Neuropathic pain induction was tested by withdrawal threshold of mechanoallodynia and thermal hyperalgesia. Immunohistochemical detection of TNFa, IL-6, and their receptors as well as IL-10 was investigated in both ipsilateral and contralateral lumbar (L4-5) and cervical (C7-8) DRG following CCI for 1, 3, 7 and 14 days. In addition, Western blot and ELISA were used for quantitative analyses and in situ hybridization to prove synthesis of cytokines. We have demonstrated that bilateral changes of cytokines in DRG are not limited only to segments associated with injured nerve but also spread alongside neuroaxis to non-associated DRG. We hypothesize that signaling for extension of neuroinflammation could be based on cytokine-cytokine interactions, and/or prostanoids and complement proteins can be involved. However, it remains to confirm what signals are responsible for propagation of neuroinflammation into DRG far from nerve injury.

Abstract (in Czech)

Walerova degenerace probíhající po mechanickém poškození periferního nervu má významnou zánětlivou komponentu (neurozánět), primárně spojenou s produkcí různých mediátorů včetně cytokinů. Immunohistochemická detekce TNFa, IL-6 a jejich receptorů, stejně jako IL-10 byla zkoumána jak u ipsi, tak i kontralaterálních lumbálních a krčních spinálních ganglií po chronické konstrikci periferního nervu. Práce ukázala, že bilaterální změny cytokinů ve spinálních gangliích nejsou omezeny jen na segmenty spojené s poškozeným nervem, ale jsou šířeny podél nervové osy i k neasociovaným gangliím.

Links

• MSM0021622404, plan (intention): Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.