2010
Disruption of retinoid signalling and differentiation process by PAHs and N-PAHs in vitro
BENÍŠEK, Martin, Luděk BLÁHA, Klára HILSCHEROVÁ a Petra KUBINCOVÁZákladní údaje
Originální název
Disruption of retinoid signalling and differentiation process by PAHs and N-PAHs in vitro
Autoři
BENÍŠEK, Martin (203 Česká republika, garant, domácí), Luděk BLÁHA (203 Česká republika, domácí), Klára HILSCHEROVÁ (203 Česká republika, domácí) a Petra KUBINCOVÁ (203 Česká republika, domácí)
Vydání
SETAC Europe, 20th Annual Meeting, Seville, Spain, 2010
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30304 Public and environmental health
Stát vydavatele
Belgie
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/10:00046568
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
retinoids; differentiation; PAHs; N-PAHs
Příznaky
Mezinárodní význam
Změněno: 10. 1. 2011 14:17, Mgr. Martin Beníšek, Ph.D.
Anotace
V originále
Polycyclic aromatic hydrocarbons (PAHs) are similar to their N-heterocyclic analogs (N-PAHs) important and widespread environmental contaminants generated especially by the incomplete combustion of organic materials. Many of them are known carcinogens, mutagens and teratogens. Teratogenicity of PAHs and N-PAHs could be caused by disruption of retinoid signaling by these compounds, as retinoid levels are crucial for differentiation process and normal embryonic development. In this study the interference of PAHs and N-PAHs with retinoid signaling system was tested using P19/A15 cell line (Approximately one half of tested compounds downregulated RA activity after 6h exposure with lower or no downregulation after 24 h. After 24 h exposure most of three, four and five ring PAHs and N-PAHs increased RA activity. Finally, P19 cells were exposed to selected active compounds either alone or in co-exposure with RA and effects on differentiation process (analysis of Oct-4 protein levels modulation) were assessed.
Návaznosti
| 2B08036, projekt VaV |
|