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@article{918494,
author = {Stejskal, Stanislav and Krontorád Koutná, Irena and Ručka, Zdeněk},
article_number = {6},
keywords = {total RNA; gradient centrifugation; cell isolation; magnetic separation; gene expression; expression activity; neutrophils; eosinophils; granulocytes},
language = {eng},
issn = {0015-5500},
journal = {FOLIA BIOLOGICA},
title = {Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?},
volume = {56},
year = {2010}
}
TY - JOUR
ID - 918494
AU - Stejskal, Stanislav - Krontorád Koutná, Irena - Ručka, Zdeněk
PY - 2010
TI - Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?
JF - FOLIA BIOLOGICA
VL - 56
IS - 6
SP - 252 - 255
EP - 252 - 255
SN - 00155500
KW - total RNA
KW - gradient centrifugation
KW - cell isolation
KW - magnetic separation
KW - gene expression
KW - expression activity
KW - neutrophils
KW - eosinophils
KW - granulocytes
N2 - Isolation of granulocyte cells from blood is necessary for accurate studying of changes in their expression. After gradient centrifugation, we obtain relatively pure granulocyte populations with different ratios of neutrophils and eosinophils. Unfortunately, in many studies in this field the expression results are not set according to the real variability of the granulocyte population. In many cases, the granulocyte population is marked simply as neutrophils and the residual population of eosinophils is not considered. Based on our recent study where we tracked the general transcription factor RNA polymerase II (RNAP II), we hypothesized that eosinophils are more transcriptionally active cells than neutrophils. We decided to test our hypothesis on isolated cells because its implications could change our view on many past expression analyses performed on granulocytes. In our experiments, we isolated neutrophils and eosinophils and measured their total RNA production. According to our results, eosinophils produce much more RNA than neutrophils. Therefore, relatively low numbers of highly active eosinophils can markedly affect the whole pool of granulocytic RNA. We want to emphasize that either a detailed description of the cell population or the use of a pure neutrophil population is necessary for the correct interpretation of neutrophil expression analysis results.
ER -
STEJSKAL, Stanislav, Irena KRONTORÁD KOUTNÁ a Zdeněk RUČKA. Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils? \textit{FOLIA BIOLOGICA}. 2010, roč.~56, č.~6, s.~252 - 255. ISSN~0015-5500.
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