"Mycobacterium avium subsp. hominissuis" in Neck Lymph Nodes of Children and their Environment Examined by Culture and Triplex Quantitative Real-Time PCR

KAEVSKA, Marija, Iva SLANÁ, Petr KRALIK, Udo REISCHL, Jaroslava OROSOVA, Alena HOLČÍKOVÁ a Ivo PAVLÍK. "Mycobacterium avium subsp. hominissuis" in Neck Lymph Nodes of Children and their Environment Examined by Culture and Triplex Quantitative Real-Time PCR. Journal of Clinical Microbiology. Washington: American Society for Microbiology, 2011, roč. 49, č. 1, s. 167-172. ISSN 0095-1137. Dostupné z: https://dx.doi.org/10.1128/JCM.00802-10.

Základní údaje

Originální název

"Mycobacterium avium subsp. hominissuis" in Neck Lymph Nodes of Children and their Environment Examined by Culture and Triplex Quantitative Real-Time PCR

Autoři

KAEVSKA, Marija (807 Severní Makedonie), Iva SLANÁ (203 Česká republika, domácí), Petr KRALIK (203 Česká republika), Udo REISCHL (276 Německo), Jaroslava OROSOVA (203 Česká republika), Alena HOLČÍKOVÁ (203 Česká republika, garant, domácí) a Ivo PAVLÍK (203 Česká republika)

Vydání

Journal of Clinical Microbiology, Washington, American Society for Microbiology, 2011, 0095-1137

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30300 3.3 Health sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.153

Kód RIV

RIV/00216224:14110/11:00051667

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1128/JCM.00802-10

UT WoS

000285787100022

Klíčová slova anglicky

Mycobacterium avium

Příznaky

Mezinárodní význam

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Anotace

V originále

“Mycobacterium avium subsp. hominissuis” often causes cervical lymphadenitis in children; its prompt and accurate identification enables adequate therapy, tracing, and prevention. The aims of this study were to determine the causative agent of lymphadenitis using culture, PCR, and triplex quantitative real-time PCR (qPCR) methods with DNA directly isolated from tissue, as well as to identify possible sources of infection from the environment. We confirmed the diagnoses by detecting M. avium subsp. hominissuis using qPCR with DNA directly isolated from lymph node biopsy specimens of two patients. In order to trace the source of infection from the environment, a method of DNA isolation from soil and other environmental samples, such as dust, cobwebs, and compost, was developed. The triplex qPCR examination revealed the presence of M. avium subsp. hominissuis in a high proportion of the environmental samples (42.8% in the first patient’s house and 47.6% in the second patient’s house). Both patients were also exposed to M. avium subsp. avium, which was present due to the breeding of infected domestic hens. The high infectious dose of M. avium subsp. hominissuis or the increased susceptibility of humans to M. avium subsp. hominissuis compared to M. avium subsp. avium could be the reason why the children were infected with M. avium subsp. hominissuis.