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@article{918770,
author = {Kaevska, Marija and Slaná, Iva and Kralik, Petr and Reischl, Udo and Orosova, Jaroslava and Holčíková, Alena and Pavlík, Ivo},
article_location = {Washington},
article_number = {1},
doi = {http://dx.doi.org/10.1128/JCM.00802-10},
keywords = {Mycobacterium avium},
language = {eng},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
title = {"Mycobacterium avium subsp. hominissuis" in Neck Lymph Nodes of Children and their Environment Examined by Culture and Triplex Quantitative Real-Time PCR},
volume = {49},
year = {2011}
}
TY - JOUR
ID - 918770
AU - Kaevska, Marija - Slaná, Iva - Kralik, Petr - Reischl, Udo - Orosova, Jaroslava - Holčíková, Alena - Pavlík, Ivo
PY - 2011
TI - "Mycobacterium avium subsp. hominissuis" in Neck Lymph Nodes of Children and their Environment Examined by Culture and Triplex Quantitative Real-Time PCR
JF - Journal of Clinical Microbiology
VL - 49
IS - 1
SP - 167-172
EP - 167-172
PB - American Society for Microbiology
SN - 00951137
KW - Mycobacterium avium
N2 - “Mycobacterium avium subsp. hominissuis” often causes cervical lymphadenitis in children; its prompt and accurate identification enables adequate therapy, tracing, and prevention. The aims of this study were to determine the causative agent of lymphadenitis using culture, PCR, and triplex quantitative real-time PCR (qPCR) methods with DNA directly isolated from tissue, as well as to identify possible sources of infection from the environment. We confirmed the diagnoses by detecting M. avium subsp. hominissuis using qPCR with DNA directly isolated from lymph node biopsy specimens of two patients. In order to trace the source of infection from the environment, a method of DNA isolation from soil and other environmental samples, such as dust, cobwebs, and compost, was developed. The triplex qPCR examination revealed the presence of M. avium subsp. hominissuis in a high proportion of the environmental samples (42.8% in the first patient’s house and 47.6% in the second patient’s house). Both patients were also exposed to M. avium subsp. avium, which was present due to the breeding of infected domestic hens. The high infectious dose of M. avium subsp. hominissuis or the increased susceptibility of humans to M. avium subsp. hominissuis compared to M. avium subsp. avium could be the reason why the children were infected with M. avium subsp. hominissuis.
ER -
KAEVSKA, Marija, Iva SLANÁ, Petr KRALIK, Udo REISCHL, Jaroslava OROSOVA, Alena HOLČÍKOVÁ a Ivo PAVLÍK. ''Mycobacterium avium subsp. hominissuis'' in Neck Lymph Nodes of Children and their Environment Examined by Culture and Triplex Quantitative Real-Time PCR. \textit{Journal of Clinical Microbiology}. Washington: American Society for Microbiology, 2011, roč.~49, č.~1, s.~167-172. ISSN~0095-1137. Dostupné z: https://dx.doi.org/10.1128/JCM.00802-10.
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