Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model

HÝŽA, Petr, Libor STREIT, Eduard GOPFERT, Daniel SCHWARZ, Michal MASAŘÍK, Michal JURAJDA, Anna VAŠKŮ a Jiří VESELÝ. Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model. ACTA VETERINARIA BRNO. VFU Brno: VFU Brno, 2010, roč. 79, č. 3, s. 453-457. ISSN 0001-7213.

Základní údaje

• Originální název: Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model
• Autoři: HÝŽA, Petr (203 Česká republika, garant, domácí), Libor STREIT (203 Česká republika, domácí), Eduard GOPFERT (203 Česká republika), Daniel SCHWARZ (203 Česká republika, domácí), Michal MASAŘÍK (203 Česká republika, domácí), Michal JURAJDA (203 Česká republika, domácí), Anna VAŠKŮ (203 Česká republika, domácí) a Jiří VESELÝ (203 Česká republika, domácí).
• Vydání: ACTA VETERINARIA BRNO, VFU Brno, VFU Brno, 2010, 0001-7213.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 30200 3.2 Clinical medicine
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 0.534
• Kód RIV: RIV/00216224:14110/10:00040874
• Organizační jednotka: Lékařská fakulta
• UT WoS: 000285269100016
• Klíčová slova anglicky: Vasospasm; pathogenesis; microsurgery; pig

Anotace

The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 was not proven

Anotace česky

The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 was not proven

Návaznosti

• NR8368, projekt VaV: Název: Spazmy cév mikrochirurgicky přenášených laloků a možnosti jejich ovlivnění

Investor: Ministerstvo zdravotnictví ČR, Spazmy cév mikrochirurgicky přenášených laloků a možnosti jejich ovlivnění