2010
Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model
HÝŽA, Petr, Libor STREIT, Eduard GOPFERT, Daniel SCHWARZ, Michal MASAŘÍK et. al.Základní údaje
Originální název
Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model
Autoři
HÝŽA, Petr (203 Česká republika, garant, domácí), Libor STREIT (203 Česká republika, domácí), Eduard GOPFERT (203 Česká republika), Daniel SCHWARZ (203 Česká republika, domácí), Michal MASAŘÍK (203 Česká republika, domácí), Michal JURAJDA (203 Česká republika, domácí), Anna VAŠKŮ (203 Česká republika, domácí) a Jiří VESELÝ (203 Česká republika, domácí)
Vydání
ACTA VETERINARIA BRNO, VFU Brno, VFU Brno, 2010, 0001-7213
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30200 3.2 Clinical medicine
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 0.534
Kód RIV
RIV/00216224:14110/10:00040874
Organizační jednotka
Lékařská fakulta
UT WoS
000285269100016
Klíčová slova anglicky
Vasospasm; pathogenesis; microsurgery; pig
Změněno: 21. 1. 2011 11:03, MUDr. Michal Jurajda, Ph.D.
V originále
The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 was not proven
Česky
The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 was not proven
Návaznosti
| NR8368, projekt VaV |
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