Parameters of oxidative stress, DNA damage and DNA repair in
type 1 and type 2 diabetes mellitus

J 2011

Parameters of oxidative stress, DNA damage and DNA repair in type 1 and type 2 diabetes mellitus

PÁCAL, Lukáš, Jana VARVAŘOVSKÁ, Zdeněk RUŠAVÝ, Silva LACIGOVÁ, Rudolf ŠTĚTINA et. al.

Základní údaje

Originální název

Parameters of oxidative stress, DNA damage and DNA repair in type 1 and type 2 diabetes mellitus

Autoři

PÁCAL, Lukáš (203 Česká republika, garant, domácí), Jana VARVAŘOVSKÁ (203 Česká republika), Zdeněk RUŠAVÝ (203 Česká republika, domácí), Silva LACIGOVÁ (203 Česká republika), Rudolf ŠTĚTINA (203 Česká republika), Jaroslav RACEK (203 Česká republika, domácí), Renata POMAHAČOVÁ (203 Česká republika), Veronika TANHÄUSEROVÁ (203 Česká republika, domácí) a Kateřina KAŇKOVÁ (203 Česká republika, domácí)

Vydání

Archives of Physiology and Biochemistry, 2011, 1381-3455

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.841 v roce 2000

Kód RIV

RIV/00216224:14110/11:00051772

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

diabetes; oxidative stress; antioxidant enzymes; DNA repair; single strand breaks

Změněno: 22. 3. 2012 15:52, Mgr. Michal Petr

Anotace

V originále

OBJECTIVES: (i) to determine the extent of oxidative stress and DNA damage and repair using a panel of selected markers in patients with type 1 and type 2 diabetes mellitus (T1DM, T2DM), (ii) to find their possible relationships with diabetes compensation and duration, and finally (iii) to test for the effect of functional polymorphisms in the 8-oxoguanin DNA glycosylase (rs1052133), catalase (rs1001179) and superoxide dismutase (rs4880) genes on respective intermediate phenotypes. METHODS: A total of 207 subjects (23 children and 44 adults with T1DM, 52 adult patients with T2DM and 88 healthy adult control subjects) were enrolled in the study. The following markers of redox state were determined in participants: erythrocyte superoxide dismutase (Ery-SOD), whole blood glutathione peroxidase (WB-GPx), erythrocyte glutathione (Ery-GSH), plasma total antioxidant capacity (P-tAOC) and plasma malondialdehyde (P-MDA). Furthermore, the extent of DNA damage and repair was ascertained using the following parameters: DNA single strand breaks (DNAssb), DNA repair capacity (DNArc) and DNA repair index (DNRI). RESULTS: Comparison of T1DM vs. T2DM patients revealed significantly higher Ery-GSH content and significantly lower Ery-SOD activity (P = 0.0006) and P-tAOC level in T1DM subjects. T2DM diabetics exhibited a significant increase in DNAssb and significant decrease in both DNArc (P < 0.0001) and DNRI compared with T1DM patients. Patient's age (irrespective of DM type) significantly correlated with DNAssb, DNArc and DNRI. Allele frequencies of all studied polymorphisms did not exhibit any significant association with the investigated parameters. CONCLUSION: We demonstrated significant age- and DM type-related changes of oxidative DNA modification and capacity for its repair in subjects with T1DM and T2DM.

Citovat

PÁCAL, Lukáš, Jana VARVAŘOVSKÁ, Zdeněk RUŠAVÝ, Silva LACIGOVÁ, Rudolf ŠTĚTINA, Jaroslav RACEK, Renata POMAHAČOVÁ, Veronika TANHÄUSEROVÁ a Kateřina KAŇKOVÁ. Parameters of oxidative stress, DNA damage and DNA repair in type 1 and type 2 diabetes mellitus. Archives of Physiology and Biochemistry. 2011, roč. 117, č. 4, s. 222-230. ISSN 1381-3455.

@article{921939,
   author = {Pácal, Lukáš and Varvařovská, Jana and Rušavý, Zdeněk and Lacigová, Silva and Štětina, Rudolf and Racek, Jaroslav and Pomahačová, Renata and Tanhäuserová, Veronika and Kaňková, Kateřina},
   article_number = {4},
   keywords = {diabetes; oxidative stress; antioxidant enzymes; DNA repair; single strand breaks},
   language = {eng},
   issn = {1381-3455},
   journal = {Archives of Physiology and Biochemistry},
   title = {Parameters of oxidative stress, DNA damage and DNA repair in type 1 and type 2 diabetes mellitus},
   url = {http://informahealthcare.com/doi/pdf/10.3109/13813455.2010.551135},
   volume = {117},
   year = {2011}
}

TY  - JOUR
ID  - 921939
AU  - Pácal, Lukáš - Varvařovská, Jana - Rušavý, Zdeněk - Lacigová, Silva - Štětina, Rudolf - Racek, Jaroslav - Pomahačová, Renata - Tanhäuserová, Veronika - Kaňková, Kateřina
PY  - 2011
TI  - Parameters of oxidative stress, DNA damage and DNA repair in type 1 and type 2 diabetes mellitus
JF  - Archives of Physiology and Biochemistry
VL  - 117
IS  - 4
SP  - 222-230
EP  - 222-230
SN  - 13813455
KW  - diabetes
KW  - oxidative stress
KW  - antioxidant enzymes
KW  - DNA repair
KW  - single strand breaks
UR  - http://informahealthcare.com/doi/pdf/10.3109/13813455.2010.551135
N2  - OBJECTIVES: (i) to determine the extent of oxidative stress and DNA damage and repair using a panel of selected markers in patients with type 1 and type 2 diabetes mellitus (T1DM, T2DM), (ii) to find their possible relationships with diabetes compensation and duration, and finally (iii) to test for the effect of functional polymorphisms in the 8-oxoguanin DNA glycosylase (rs1052133), catalase (rs1001179) and superoxide dismutase (rs4880) genes on respective intermediate phenotypes. METHODS: A total of 207 subjects (23 children and 44 adults with T1DM, 52 adult patients with T2DM and 88 healthy adult control subjects) were enrolled in the study. The following markers of redox state were determined in participants: erythrocyte superoxide dismutase (Ery-SOD), whole blood glutathione peroxidase (WB-GPx), erythrocyte glutathione (Ery-GSH), plasma total antioxidant capacity (P-tAOC) and plasma malondialdehyde (P-MDA). Furthermore, the extent of DNA damage and repair was ascertained using the following parameters: DNA single strand breaks (DNAssb), DNA repair capacity (DNArc) and DNA repair index (DNRI). RESULTS: Comparison of T1DM vs. T2DM patients revealed significantly higher Ery-GSH content and significantly lower Ery-SOD activity (P = 0.0006) and P-tAOC level in T1DM subjects. T2DM diabetics exhibited a significant increase in DNAssb and significant decrease in both DNArc (P < 0.0001) and DNRI compared with T1DM patients. Patient's age (irrespective of DM type) significantly correlated with DNAssb, DNArc and DNRI. Allele frequencies of all studied polymorphisms did not exhibit any significant association with the investigated parameters. CONCLUSION: We demonstrated significant age- and DM type-related changes of oxidative DNA modification and capacity for its repair in subjects with T1DM and T2DM.
ER  -

PÁCAL, Lukáš, Jana VARVAŘOVSKÁ, Zdeněk RUŠAVÝ, Silva LACIGOVÁ, Rudolf ŠTĚTINA, Jaroslav RACEK, Renata POMAHAČOVÁ, Veronika TANHÄUSEROVÁ a Kateřina KAŇKOVÁ. Parameters of oxidative stress, DNA damage and DNA repair in type 1 and type 2 diabetes mellitus. \textit{Archives of Physiology and Biochemistry}. 2011, roč.~117, č.~4, s.~222-230. ISSN~1381-3455.

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