Avidin and plant biotechnology to control pests

C 2010

Avidin and plant biotechnology to control pests

MARTIN, Harry, Elisabeth BURGESS, Michal MASAŘÍK, Karl KRAMER, Miroslava BEKLOVÁ et. al.

Základní údaje

Originální název

Avidin and plant biotechnology to control pests

Autoři

MARTIN, Harry (840 Spojené státy), Elisabeth BURGESS (36 Austrálie), Michal MASAŘÍK (203 Česká republika), Karl KRAMER (840 Spojené státy), Miroslava BEKLOVÁ (203 Česká republika, domácí), Vojtěch ADAM (203 Česká republika, domácí) a René KIZEK (203 Česká republika, garant, domácí)

Vydání

1st Edition. New York, Genetic Engineering, Biofertilisation, Soil Quality and Organic Farming, od s. 1-22, 22 s. Sustainable Agriculture Reviews Volume 4, 2010

Nakladatel

Springer

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/10:00051778

Organizační jednotka

Lékařská fakulta

ISBN

978-90-481-8740-9

Klíčová slova anglicky

Transgenic plants; avidinbiotin technology; agriculture; electrochemical method

Příznaky

Mezinárodní význam

Změněno: 5. 4. 2012 16:12, Mgr. Michal Petr

Anotace

V originále

In this review, we discuss the application of transgenic avidin, a protein naturally occurring in eggwhite, in the protection of rice, maize, potato and apple leaf from insect pests. Avidin binds the vitamin, biotin with extraordinary affinity (10minus15 M). Biotin is a watersoluble vitamin that is required for normal cellular metabolism and growth. The presence of avidin in the diet of insect pests is lethal since biotin is unavailable to them. The use of streptavidin, a bacterial homologue of avidin, is also described. We discuss the subcellular targeting of avidin expression in plants to avoid toxicity to the plant host and we describe the qualities of avidin which make it suitable for crop protection during cultivation and storage. Avidin is stable under normal conditions of crop storage but biodegradable and destroyed by cooking. These combined qualities make it an excellent choice for the protection of crops from insects. Finally, we discuss the modification of the avidin gene to allow expression in plants, the methods for transfection of the gene into plants, and the approaches used to quantify gene expression and avidin function in plant tissues. These methods include: polymerase chain reaction; enzymelinked immmunosorbent assay; polyacrylamide gelelectrophoresis; fluorescence polarisation (FP); capillary electrophoresis; tissueprinting; square-wave voltammetry (SWV) and the measurement of larvae morbidity and mortality.

Citovat

MARTIN, Harry, Elisabeth BURGESS, Michal MASAŘÍK, Karl KRAMER, Miroslava BEKLOVÁ, Vojtěch ADAM a René KIZEK. Avidin and plant biotechnology to control pests. In Genetic Engineering, Biofertilisation, Soil Quality and Organic Farming. 1st Edition. New York: Springer, 2010, s. 1-22. Sustainable Agriculture Reviews Volume 4. ISBN 978-90-481-8740-9.

@inbook{922230,
   author = {Martin, Harry and Burgess, Elisabeth and Masařík, Michal and Kramer, Karl and Beklová, Miroslava and Adam, Vojtěch and Kizek, René},
   address = {New York},
   booktitle = {Genetic Engineering, Biofertilisation, Soil Quality and Organic Farming},
   edition = {1st Edition},
   keywords = {Transgenic plants; avidinbiotin technology; agriculture; electrochemical method},
   language = {eng},
   location = {New York},
   isbn = {978-90-481-8740-9},
   pages = {1-22},
   publisher = {Springer},
   title = {Avidin and plant biotechnology to control pests},
   year = {2010}
}

TY  - CHAP
ID  - 922230
AU  - Martin, Harry - Burgess, Elisabeth - Masařík, Michal - Kramer, Karl - Beklová, Miroslava - Adam, Vojtěch - Kizek, René
PY  - 2010
TI  - Avidin and plant biotechnology to control pests
VL  - Sustainable Agriculture Reviews Volume 4
PB  - Springer
CY  - New York
SN  - 9789048187409
KW  - Transgenic plants
KW  - avidinbiotin technology
KW  - agriculture
KW  - electrochemical method
N2  - In this review, we discuss the application of transgenic avidin, a protein naturally occurring in eggwhite, in the protection of rice, maize, potato and apple leaf from insect pests. Avidin binds the vitamin, biotin with extraordinary affinity (10minus15 M). Biotin is a watersoluble vitamin that is required for normal cellular metabolism and growth. The presence of avidin in the diet of insect pests is lethal since biotin is unavailable to them. The use of streptavidin, a bacterial homologue of avidin, is also described. We discuss the subcellular targeting of avidin expression in plants to avoid toxicity to the plant host and we describe the qualities of avidin which make it suitable for crop protection during cultivation and storage. Avidin is stable under normal conditions of crop storage but biodegradable and destroyed by cooking. These combined qualities make it an excellent choice for the protection of crops from insects. Finally, we discuss the modification of the avidin gene to allow expression in plants, the methods for transfection of the gene into plants, and the approaches used to quantify gene expression and avidin function in plant tissues. These methods include: polymerase chain reaction; enzymelinked immmunosorbent assay; polyacrylamide gelelectrophoresis; fluorescence polarisation (FP); capillary electrophoresis; tissueprinting; square-wave voltammetry (SWV) and the measurement of larvae morbidity and mortality.
ER  -

MARTIN, Harry, Elisabeth BURGESS, Michal MASAŘÍK, Karl KRAMER, Miroslava BEKLOVÁ, Vojtěch ADAM a René KIZEK. Avidin and plant biotechnology to control pests. In \textit{Genetic Engineering, Biofertilisation, Soil Quality and Organic Farming}. 1st Edition. New York: Springer, 2010, s.~1-22. Sustainable Agriculture Reviews Volume 4. ISBN~978-90-481-8740-9.

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