Increasing hematopoietic microchimerism is a reliable indicator
of incipient AML relapse

J 2010

Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse

HORKÝ, Ondřej, Jiří MAYER, Lenka KABLÁSKOVÁ, Filip RÁZGA, Marta KREJČÍ et. al.

Základní údaje

Originální název

Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse

Autoři

HORKÝ, Ondřej (203 Česká republika, garant, domácí), Jiří MAYER (203 Česká republika, domácí), Lenka KABLÁSKOVÁ (203 Česká republika, domácí), Filip RÁZGA (703 Slovensko, domácí), Marta KREJČÍ (203 Česká republika, domácí), Jarmila KISSOVÁ (203 Česká republika, domácí), Marek BORSKÝ (203 Česká republika, domácí), Ivana JEŽÍŠKOVÁ (203 Česká republika) a Dana DVOŘÁKOVÁ (203 Česká republika, domácí)

Vydání

International Journal of Laboratory Hematology, 2010, 1751-5521

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.368

Kód RIV

RIV/00216224:14110/10:00047063

Organizační jednotka

Lékařská fakulta

UT WoS

000286052900006

Klíčová slova anglicky

microchimerism; chimerism; real-time PCR; relapse; acute myeloid leukemia

Změněno: 10. 5. 2011 08:45, Mgr. Ondřej Horký, Ph.D.

Anotace

V originále

Using real-time PCR (RQ-PCR), we compare peripheral blood samples obtained from patients with acute myeloid leukemia (AML) before hematological relapse and those taken during complete remission. By comparison of these two groups, we describe microchimerism dynamics clearly connected with imminent AML relapse. Mere reappearance of autologous hematopoiesis within patients with complete donor chimerism is alarming, and another sample with further increase confirms ongoing relapse. In case of patients with continuous microchimerism, another two consecutive samples with increasing trend are required. RQ-PCR predicted a significantly higher number of hematological relapses (87% vs. 39%) with a median anticipation period of 33 days, 26 days earlier than conventional PCR (P = 0.0002). Moreover, the outcome of microchimerism dynamics was in complete agreement with monitoring of minimal residual disease when analyzed from the same cell compartment.

Návaznosti

MSM0021622430, záměr

Název: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Citovat

HORKÝ, Ondřej, Jiří MAYER, Lenka KABLÁSKOVÁ, Filip RÁZGA, Marta KREJČÍ, Jarmila KISSOVÁ, Marek BORSKÝ, Ivana JEŽÍŠKOVÁ a Dana DVOŘÁKOVÁ. Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse. International Journal of Laboratory Hematology. 2010, roč. 33, č. 1, 10 s. ISSN 1751-5521.

@article{922804,
   author = {Horký, Ondřej and Mayer, Jiří and Kablásková, Lenka and Rázga, Filip and Krejčí, Marta and Kissová, Jarmila and Borský, Marek and Ježíšková, Ivana and Dvořáková, Dana},
   article_number = {1},
   keywords = {microchimerism; chimerism; real-time PCR; relapse; acute myeloid leukemia},
   language = {eng},
   issn = {1751-5521},
   journal = {International Journal of Laboratory Hematology},
   title = {Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse},
   volume = {33},
   year = {2010}
}

TY  - JOUR
ID  - 922804
AU  - Horký, Ondřej - Mayer, Jiří - Kablásková, Lenka - Rázga, Filip - Krejčí, Marta - Kissová, Jarmila - Borský, Marek - Ježíšková, Ivana - Dvořáková, Dana
PY  - 2010
TI  - Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse
JF  - International Journal of Laboratory Hematology
VL  - 33
IS  - 1
SN  - 17515521
KW  - microchimerism
KW  - chimerism
KW  - real-time PCR
KW  - relapse
KW  - acute myeloid leukemia
N2  - Using real-time PCR (RQ-PCR), we compare peripheral blood samples obtained from patients with acute myeloid leukemia (AML) before hematological relapse and those taken during complete remission. By comparison of these two groups, we describe microchimerism dynamics clearly connected with imminent AML relapse. Mere reappearance of autologous hematopoiesis within patients with complete donor chimerism is alarming, and another sample with further increase confirms ongoing relapse. In case of patients with continuous microchimerism, another two consecutive samples with increasing trend are required. RQ-PCR predicted a significantly higher number of hematological relapses (87% vs. 39%) with a median anticipation period of 33 days, 26 days earlier than conventional PCR (P = 0.0002). Moreover, the outcome of microchimerism dynamics was in complete agreement with monitoring of minimal residual disease when analyzed from the same cell compartment.
ER  -

HORKÝ, Ondřej, Jiří MAYER, Lenka KABLÁSKOVÁ, Filip RÁZGA, Marta KREJČÍ, Jarmila KISSOVÁ, Marek BORSKÝ, Ivana JEŽÍŠKOVÁ a Dana DVOŘÁKOVÁ. Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse. \textit{International Journal of Laboratory Hematology}. 2010, roč.~33, č.~1, 10 s. ISSN~1751-5521.

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