| SOUSA, Kyle, Carlos VILLAESCUSA, Lukáš ČAJÁNEK, Jennifer ONDR, Goncalo CASTELO-BRANCO, Wytske HOFSTRA, Vítězslav BRYJA, Carina PALMBERG, Tomas BERGMAN, Brandon WAINWRIGHT, Richard LANG and Ernest ARENAS. Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2010, vol. 285, No 10, p. 7246-7253. ISSN 0021-9258. Available from: https://dx.doi.org/10.1074/jbc.M109.079822. |
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@article{929233,
author = {Sousa, Kyle and Villaescusa, Carlos and Čajánek, Lukáš and Ondr, Jennifer and CasteloandBranco, Goncalo and Hofstra, Wytske and Bryja, Vítězslav and Palmberg, Carina and Bergman, Tomas and Wainwright, Brandon and Lang, Richard and Arenas, Ernest},
article_location = {Bethesda, USA},
article_number = {10},
doi = {http://dx.doi.org/10.1074/jbc.M109.079822},
keywords = {Wnt2; dopaminergic neuron; ventral midbrain},
language = {eng},
issn = {0021-9258},
journal = {Journal of Biological Chemistry},
title = {Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain},
volume = {285},
year = {2010}
}
TY - JOUR
ID - 929233
AU - Sousa, Kyle - Villaescusa, Carlos - Čajánek, Lukáš - Ondr, Jennifer - Castelo-Branco, Goncalo - Hofstra, Wytske - Bryja, Vítězslav - Palmberg, Carina - Bergman, Tomas - Wainwright, Brandon - Lang, Richard - Arenas, Ernest
PY - 2010
TI - Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain
JF - Journal of Biological Chemistry
VL - 285
IS - 10
SP - 7246-7253
EP - 7246-7253
PB - Amer. Soc. Biochem. Mol. Biol.
SN - 00219258
KW - Wnt2
KW - dopaminergic neuron
KW - ventral midbrain
N2 - Wnts are secreted, lipidated proteins that regulate multiple aspects of brain development, including dopaminergic neuron development. In this study, we perform the first purification and signaling analysis of Wnt2 and define the function of Wnt2 in ventral midbrain precursor cultures, as well as in Wnt2-null mice in vivo. We found that purified Wnt2 induces the phosphorylation of both Lrp5/6 and Dvl-2/3, and activates beta-catenin in SN4741 dopaminergic cells. Moreover, purified Wnt2 increases progenitor proliferation, and the number of dopaminergic neurons in ventral midbrain precursor cultures. In agreement with these findings, analysis of the ventral midbrain of developing Wnt2-null mice revealed a decrease in progenitor proliferation and neurogenesis that lead to a decrease in the number of postmitotic precursors and dopaminergic neurons. Collectively, our observations identify Wnt2 as a novel regulator of dopaminergic progenitors and dopaminergic neuron development.
ER -
SOUSA, Kyle, Carlos VILLAESCUSA, Lukáš ČAJÁNEK, Jennifer ONDR, Goncalo CASTELO-BRANCO, Wytske HOFSTRA, Vítězslav BRYJA, Carina PALMBERG, Tomas BERGMAN, Brandon WAINWRIGHT, Richard LANG and Ernest ARENAS. Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2010, vol.~285, No~10, p.~7246-7253. ISSN~0021-9258. Available from: https://dx.doi.org/10.1074/jbc.M109.079822.
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