Multilocus PCR typing strategy for differentiation of
Staphylococcus aureus siphoviruses reflecting their modular
genome structure

J 2010

Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure

KAHÁNKOVÁ, Jana, Roman PANTŮČEK, Christiane GOERKE, Vladislava RŮŽIČKOVÁ, Pavla HOLOCHOVÁ et. al.

Basic information

Original name

Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure

Authors

KAHÁNKOVÁ, Jana (203 Czech Republic, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, guarantor, belonging to the institution), Christiane GOERKE (276 Germany), Vladislava RŮŽIČKOVÁ (203 Czech Republic, belonging to the institution), Pavla HOLOCHOVÁ (203 Czech Republic, belonging to the institution) and Jiří DOŠKAŘ (203 Czech Republic, belonging to the institution)

Edition

Environmental Microbiology, UK, Wiley-Blackwell Publishing, Inc. 2010, 1462-2912

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Abstract PDF

Impact factor

Impact factor: 5.537

RIV identification code

RIV/00216224:14310/10:00047719

Organization unit

Faculty of Science

UT WoS

000281556900013

Keywords (in Czech)

bakteriofág; Staphylococcus aureus; Siphoviridae; molekularní typizace; PCR; modulární genom; evoluce

Keywords in English

bacteriophage; Staphylococcus aureus; Siphoviridae; molecular typing; PCR; modular genome; evolution

Abstract

V originále

Given the great biological importance and high diversity of temperate Staphylococcus aureus bacteriophages, a method is needed for the description of their genomic structure. Here we have updated a multiplex PCR strategy for the complex characterization of S. aureus phages of the family Siphoviridae. Based on the comparative genomic analysis of the available phage sequences, a multilocus PCR strategy for typing the major modules of the phage genome was designed. The genomic modules were classified on the basis of the genes for integrase, anti-repressor, replication proteins, dUTPase, portal protein, tail appendices and endolysin corresponding to main functional modules. The nine PCR assays designed for the above sequences were shown to be capable to identify the bacteriophage gene pool present both in the phage and bacterial genomes and their mosaic structure. The established multiplex PCR-based multilocus diagnostic scheme is convenient for rapid and reliable phage classification and for the study of bacteriophage evolution.

Links

GA310/09/0459, research and development project

Name: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

Investor: Czech Science Foundation, Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

KAHÁNKOVÁ, Jana, Roman PANTŮČEK, Christiane GOERKE, Vladislava RŮŽIČKOVÁ, Pavla HOLOCHOVÁ and Jiří DOŠKAŘ. Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure. Environmental Microbiology. UK: Wiley-Blackwell Publishing, Inc., 2010, vol. 12, No 9, p. 2527-2538. ISSN 1462-2912.

@article{933317,
   author = {Kahánková, Jana and Pantůček, Roman and Goerke, Christiane and Růžičková, Vladislava and Holochová, Pavla and Doškař, Jiří},
   article_location = {UK},
   article_number = {9},
   keywords = {bacteriophage; Staphylococcus aureus; Siphoviridae; molecular typing; PCR; modular genome; evolution},
   language = {eng},
   issn = {1462-2912},
   journal = {Environmental Microbiology},
   title = {Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2010.02226.x/abstract},
   volume = {12},
   year = {2010}
}

TY  - JOUR
ID  - 933317
AU  - Kahánková, Jana - Pantůček, Roman - Goerke, Christiane - Růžičková, Vladislava - Holochová, Pavla - Doškař, Jiří
PY  - 2010
TI  - Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure
JF  - Environmental Microbiology
VL  - 12
IS  - 9
SP  - 2527-2538
EP  - 2527-2538
PB  - Wiley-Blackwell Publishing, Inc.
SN  - 14622912
KW  - bacteriophage
KW  - Staphylococcus aureus
KW  - Siphoviridae
KW  - molecular typing
KW  - PCR
KW  - modular genome
KW  - evolution
UR  - http://onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2010.02226.x/abstract
N2  - Given the great biological importance and high diversity of temperate Staphylococcus aureus bacteriophages, a method is needed for the description of their genomic structure. Here we have updated a multiplex PCR strategy for the complex characterization of S. aureus phages of the family Siphoviridae. Based on the comparative genomic analysis of the available phage sequences, a multilocus PCR strategy for typing the major modules of the phage genome was designed. The genomic modules were classified on the basis of the genes for integrase, anti-repressor, replication proteins, dUTPase, portal protein, tail appendices and endolysin corresponding to main functional modules. The nine PCR assays designed for the above sequences were shown to be capable to identify the bacteriophage gene pool present both in the phage and bacterial genomes and their mosaic structure. The established multiplex PCR-based multilocus diagnostic scheme is convenient for rapid and reliable phage classification and for the study of bacteriophage evolution.
ER  -

KAHÁNKOVÁ, Jana, Roman PANTŮČEK, Christiane GOERKE, Vladislava RŮŽIČKOVÁ, Pavla HOLOCHOVÁ and Jiří DOŠKAŘ. Multilocus PCR typing strategy for differentiation of Staphylococcus aureus siphoviruses reflecting their modular genome structure. \textit{Environmental Microbiology}. UK: Wiley-Blackwell Publishing, Inc., 2010, vol.~12, No~9, p.~2527-2538. ISSN~1462-2912.

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