| PŘIBYL, Jan, Petr SKLÁDAL, Daniel RENČIUK a Michaela VORLÍČKOVÁ. High resolution AFM as a tool for analysis of short-chain DNA structure and properties. In Biosurf 2007, Zurich. Zurich: ETH Zurich, CH, 2007, s. 41-42, 150 s. ISSN 1473-2262. |
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@inproceedings{937677,
author = {Přibyl, Jan and Skládal, Petr and Renčiuk, Daniel and Vorlíčková, Michaela},
address = {Zurich},
booktitle = {Biosurf 2007, Zurich},
keywords = {AFM microscope DNA mica HOPG},
language = {eng},
location = {Zurich},
pages = {41-42},
publisher = {ETH Zurich, CH},
title = {High resolution AFM as a tool for analysis of short-chain DNA structure and properties},
year = {2007}
}
TY - JOUR
ID - 937677
AU - Přibyl, Jan - Skládal, Petr - Renčiuk, Daniel - Vorlíčková, Michaela
PY - 2007
TI - High resolution AFM as a tool for analysis of short-chain DNA structure and properties
PB - ETH Zurich, CH
CY - Zurich
KW - AFM microscope DNA mica HOPG
N2 - Immobilization of biomolecules on surfaces while keeping the maximum conformation flexibility of the molecules is one of the most important techniques for atomic force microscopy imaging. When the DNA molecules are studied withAtomic Force Microscope (AFM) either HOPG (Highly Ordered Pyrolytic Graphite) or freshly cleaved mica is used as asubstrate because they show atomically flat surface. DNA molecules can be adsorbed to the graphite surface, howeverthis kind of supporting material is very expensive. Mica shows high flatness, unfortunately it can not be used for for DNAimmobilization without specific treatment, as both mica and DNA are negatively charged in standard buffer solutions.Scanning probe microscopy (SPM) are based on the ability to detect a local property of a surface by means of spatiallycontrolled sensor or probe. Typically, the sensor is scanned over the area of interest so it is only transiently localized inthe neighbourhood of a given point on the surface.
ER -
PŘIBYL, Jan, Petr SKLÁDAL, Daniel RENČIUK a Michaela VORLÍČKOVÁ. High resolution AFM as a tool for analysis of short-chain DNA structure and properties. In \textit{Biosurf 2007, Zurich}. Zurich: ETH Zurich, CH, 2007, s.~41-42, 150 s. ISSN~1473-2262.
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