2008
Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization.
KŘÍŽ, Martin, Jan PŘIBYL, Petr SKLÁDAL a Miloslav SUCHÁNEKZákladní údaje
Originální název
Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization.
Autoři
KŘÍŽ, Martin (203 Česká republika, domácí), Jan PŘIBYL (203 Česká republika, garant, domácí), Petr SKLÁDAL (203 Česká republika, domácí) a Miloslav SUCHÁNEK (203 Česká republika)
Vydání
Brno, 5th international interdisciplinary meeting on bioanalysis CECE 2008, od s. 65-66, 2 s. 2008
Nakladatel
Institute of Analytical Chemisty AS CR
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/08:00052276
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-80-254-3194-8
Klíčová slova anglicky
AFM microscope cantilever tip silanization hydrophobicity
Změněno: 2. 4. 2012 10:53, Mgr. Jan Přibyl, Ph.D.
Anotace
V originále
Atomic Force Microscopy (AFM) technique allows visualization of structures in a sub-nanometer scale, i.e. imaging of individual biomolecules. In principle AFM scanning probe, presented by ultra-sharp tip, is in close contact with surface and relief is visualized as 3D map (after conversion of a measured quantity, e.g. tip bending). Resolution of an AFM microscope is essentially affected by the tip curvature (sharpness), however other experimental conditions would affect this parameter, too. Only weak and medium forces are usually employed in the interaction between tip and surface in the relief visualization. Surfaces visualized in a non-controlled atmosphere are naturally coated with a very thin (1-5 nm) layer of adsorbed water. In some cases capillary forces originating from this layer can strongly affect visualization process, as those forces are much stronger comparing to other intermolecular forces (van derWaals, hydrogen, ionic forces, etc.). Formation of capillary forces can be effectively suppressed by chemical modification of tip (mostly by hydrophobization of the tip) surface. Various methods for tip surface modification are presented. Procedure always starts with the surface activation by a silanization. Subsequent coupling of either hydrophilic or hydrophobic molecules determines surface properties in this way. Impact of the tip surface modification on resolution of images of individual biomolecules (proteins, nucleic acids) are shown as examples (on the right: IgG molecule-hydrophilic tip, DNA-lipophilic tip).
Návaznosti
2B06056, projekt VaV |
|