Nanopolyperoxidase – a universal system for simultaneous
colorimetric signal enhancement and nanoscale visualization of
antibody labeling

D 2008

Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling

PŘIBYL, Jan, Kateřina FRNKOVÁ, Petr SKLÁDAL a Miloslav SUCHÁNEK

Základní údaje

Originální název

Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling

Autoři

PŘIBYL, Jan (203 Česká republika, garant), Kateřina FRNKOVÁ (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí) a Miloslav SUCHÁNEK (203 Česká republika)

Vydání

Brno, 5th international interdisciplinary meeting on bioanalysis CECE 2008, od s. 70-71, 2 s. 2008

Nakladatel

Institute of Analytical Chemisty AS CR

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/08:00052277

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-254-3194-8

Klíčová slova anglicky

antibody enzyme-label blotting ELISA

Štítky

Flow through assay, Pb, rivok

Změněno: 2. 4. 2012 10:58, Mgr. Jan Přibyl, Ph.D.

Anotace

V originále

During past decades, a variety of signal amplification approaches in heterogeneous affinity immunoassays (including ELISA) has been developed. Those procedures are usually based either on recirculation enzymatic or repeated multiplication of an enzyme label via avidin-biotin system. From practical point of view, the multiplication procedure should not change experimental part of method, e.g. number of steps in procedure or solution content should not be changed. For this reason, an idea of nanopolyperoxidase (NPP) has originated. The principle is simple: gold nanoparticles are modified with an antibody as well as with labeling enzyme (Horseradish peroxidase; ration 2:8). Modified nanoparticle is stabilized in a PEG solution. Prepared NPP was studied using various methods. Atomic Force Microscopy (AFM) was used for characterization of structure and morphology in a nanometer scale (see image below). Use of NPP in construction of competitive ELISA as well as in visualization of a protein (Human Serum Albumin as model compound) on a blotting membrane is presented.

Návaznosti

2B06056, projekt VaV

Název: Diagnostika poškození DNA polyaromatickými sloučeninami použitím nanotechnologických a bioanalytických metod pro včasnou detekci karcinomu.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diagnostika poškození DNA polyaromatickými sloučeninami použitím nanotechnologických a bioanalytických metod pro včasnou detekci karcinomu

Citovat

PŘIBYL, Jan, Kateřina FRNKOVÁ, Petr SKLÁDAL a Miloslav SUCHÁNEK. Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling. In 5th international interdisciplinary meeting on bioanalysis CECE 2008. Brno: Institute of Analytical Chemisty AS CR, 2008, s. 70-71. ISBN 978-80-254-3194-8.

@inproceedings{937683,
   author = {Přibyl, Jan and Frnková, Kateřina and Skládal, Petr and Suchánek, Miloslav},
   address = {Brno},
   booktitle = {5th international interdisciplinary meeting on bioanalysis CECE 2008},
   keywords = {antibody enzyme-label blotting ELISA},
   language = {eng},
   location = {Brno},
   isbn = {978-80-254-3194-8},
   pages = {70-71},
   publisher = {Institute of Analytical Chemisty AS CR},
   title = {Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling},
   year = {2008}
}

TY  - JOUR
ID  - 937683
AU  - Přibyl, Jan - Frnková, Kateřina - Skládal, Petr - Suchánek, Miloslav
PY  - 2008
TI  - Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling
PB  - Institute of Analytical Chemisty AS CR
CY  - Brno
SN  - 9788025431948
KW  - antibody enzyme-label blotting ELISA
N2  - During past decades, a variety of signal amplification approaches in heterogeneous affinity immunoassays (including ELISA) has been developed. Those procedures are usually based either on recirculation enzymatic or repeated multiplication of an enzyme label via avidin-biotin system. From practical point of view, the multiplication procedure should not change experimental part of method, e.g. number of steps in procedure or solution content should not be changed. For this reason, an idea of nanopolyperoxidase (NPP) has originated. The principle is simple: gold nanoparticles are modified with an antibody as well as with labeling enzyme (Horseradish peroxidase; ration 2:8). Modified nanoparticle is stabilized in a PEG solution. Prepared NPP was studied using various methods. Atomic Force Microscopy (AFM) was used for characterization of structure and morphology in a nanometer scale (see image below). Use of NPP in construction of competitive ELISA as well as in visualization of a protein (Human Serum Albumin as model compound) on a blotting membrane is presented.
ER  -

PŘIBYL, Jan, Kateřina FRNKOVÁ, Petr SKLÁDAL a Miloslav SUCHÁNEK. Nanopolyperoxidase – a universal system for simultaneous colorimetric signal enhancement and nanoscale visualization of antibody labeling. In \textit{5th international interdisciplinary meeting on bioanalysis CECE 2008}. Brno: Institute of Analytical Chemisty AS CR, 2008, s.~70-71. ISBN~978-80-254-3194-8.

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