Detailed Information on Publication Record
2011
SPECTROPHOTOMETRIC ACID-BASE CHARACTERIZATION OF ADENINE ANALOGUES
BUČEK, Pavel, Přemysl LUBAL, Petra NETROUFALOVÁ and Libuše TRNKOVÁBasic information
Original name
SPECTROPHOTOMETRIC ACID-BASE CHARACTERIZATION OF ADENINE ANALOGUES
Authors
BUČEK, Pavel (203 Czech Republic, belonging to the institution), Přemysl LUBAL (203 Czech Republic, guarantor, belonging to the institution), Petra NETROUFALOVÁ (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution)
Edition
Brno, XI. Pracovní setkání fyzikálních chemiků a elektrochemiků, p. 59-61, 3 pp. 2011
Publisher
Ediční středisko Mendelu v Brně
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/11:00052420
Organization unit
Faculty of Science
ISBN
978-80-7375-514-0
Keywords in English
purine based;RNA;DNA;adenine;guanine;aminopurines;acid-based properties;UV-Vis spectroscopy;fluorescence
Změněno: 20/3/2012 14:26, Mgr. Sylvie Dohnalíková
Abstract
V originále
Purine based DNA/RNA bases adenine (6-aminopurine) and guanine (2-aminopurin-6- one), serve in live bodies as building blocks of high physiological importance. For in vitro research applications they are replaced by 2-aminopurine and 2,6-diaminopurine. These aminopurines are used either “free” as replacements for adenine/guanine in nucleic acid strands or are subject to further derivatization and use (e.g. enzyme function research [1]). Despite their frequent use, very few is known about the substances’ physico-chemical properties. We studied acid-base properties of 2-aminopurine and 2,6-diaminopurine with use of advanced chemometric tools applied to UV/Vis spectroscopy data and since the 2- aminopurine is highly fluorescent, fluorescence spectroscopy data, too.
Links
LC06035, research and development project |
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ME09065, research and development project |
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MUNI/A/0992/2009, interní kód MU |
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