D 2011

SPECTROPHOTOMETRIC ACID-BASE CHARACTERIZATION OF ADENINE ANALOGUES

BUČEK, Pavel, Přemysl LUBAL, Petra NETROUFALOVÁ and Libuše TRNKOVÁ

Basic information

Original name

SPECTROPHOTOMETRIC ACID-BASE CHARACTERIZATION OF ADENINE ANALOGUES

Authors

BUČEK, Pavel (203 Czech Republic, belonging to the institution), Přemysl LUBAL (203 Czech Republic, guarantor, belonging to the institution), Petra NETROUFALOVÁ (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Brno, XI. Pracovní setkání fyzikálních chemiků a elektrochemiků, p. 59-61, 3 pp. 2011

Publisher

Ediční středisko Mendelu v Brně

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/11:00052420

Organization unit

Faculty of Science

ISBN

978-80-7375-514-0

Keywords in English

purine based;RNA;DNA;adenine;guanine;aminopurines;acid-based properties;UV-Vis spectroscopy;fluorescence

Tags

Změněno: 20/3/2012 14:26, Mgr. Sylvie Dohnalíková

Abstract

V originále

Purine based DNA/RNA bases adenine (6-aminopurine) and guanine (2-aminopurin-6- one), serve in live bodies as building blocks of high physiological importance. For in vitro research applications they are replaced by 2-aminopurine and 2,6-diaminopurine. These aminopurines are used either “free” as replacements for adenine/guanine in nucleic acid strands or are subject to further derivatization and use (e.g. enzyme function research [1]). Despite their frequent use, very few is known about the substances’ physico-chemical properties. We studied acid-base properties of 2-aminopurine and 2,6-diaminopurine with use of advanced chemometric tools applied to UV/Vis spectroscopy data and since the 2- aminopurine is highly fluorescent, fluorescence spectroscopy data, too.

Links

LC06035, research and development project
Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.
Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
ME09065, research and development project
Name: Výzkum nových detekčních systémů na bázi senzorových polí pro použití ve speciační analýze
Investor: Ministry of Education, Youth and Sports of the CR, Research of new detection systems based on sensor arrays for application in speciation analysis, Research and Development Programme KONTAKT (ME)
MUNI/A/0992/2009, interní kód MU
Name: Vývoj metodologie a instrumentace analytické chemie pro studium složení a vlastností přírodních a syntetických materiálů (Acronym: MIA)
Investor: Masaryk University, Category A