2011
Comparison of multiplex PCRs, (GTG)5-PCR and automated ribotyping for identification of Lactobacillus spp. isolated from children's intestinal tissues.
ŠVEC, Pavel, Vladimír DRÁB, Andrea TESHIM, Lucie VOLNÁ, Ondrej ŠEDO et. al.Základní údaje
Originální název
Comparison of multiplex PCRs, (GTG)5-PCR and automated ribotyping for identification of Lactobacillus spp. isolated from children's intestinal tissues.
Autoři
ŠVEC, Pavel (203 Česká republika, garant, domácí), Vladimír DRÁB (203 Česká republika), Andrea TESHIM (203 Česká republika, domácí), Lucie VOLNÁ (203 Česká republika, domácí), Ondrej ŠEDO (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí) a Ivo SEDLÁČEK (203 Česká republika, domácí)
Vydání
Inaugural Meeting of Bergey's International Society for Microbial Systematics (BISMiS 2011). Peking, Čína, 19.-23.5. 2011. 2011
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/11:00052478
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
PCRs; automated ribotyping; Lactobacillus
Změněno: 9. 6. 2011 10:51, doc. RNDr. Pavel Švec, Ph.D.
Anotace
V originále
The genus Lactobacillus represents taxonomically diverse group of organisms inhabiting a variety of different environments including the human body. Lactobacilli are generally recognised as most important agents playing beneficial role in balancing microflora of vagina and gastrointestinal tract. This study deals with a group of Lactobacillus strains isolated from the biopsy samples retrieved from children's intestinal tissues. All strains were identified using the Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (Ultraflex III instrument, Bruker Daltonik) and assigned as L. casei/paracasei (11 strains), L. rhamnosus (7), L. mucosae (5), L. salivarius (3), L. gasseri (2), L. plantarum (2), and L. oris (1). Investigated group was further subjected to the multiplex PCR assays based on the integrated sequences of 16S and 23S rRNA genes, (GTG)5-PCR fingerprinting performed by the DiversiLab system (bioMérieux) and automated ribotyping with the RiboPrinter identification system (DuPont Qualicon). Multiplex PCRs identified correctly all L. plantarum, L. rhamnosus and L. salivarius strains, ten L. casei/paracasei, and one L. gasseri strain. Similar results were obtained using (GTG)5-PCR fingerprinting which assigned correctly all L. casei/paracasei, L. gasseri, L. oris, L. rhamnosus and L. salivarius strains. Automatic identification procedure performed by the RiboPrinter system identified only L. casei/paracasei strains, two L. salivarius and one L. gasseri. In conclusion, multiplex PCR assays and (GTG)5-PCR fingerprinting were revealed as good tools for the identification of the investigated intestinal lactobacilli. In contrast, the successfulness of the automatic identification procedure performed by the RiboPrinter system was low. This work was supported by grants 2B08068, LC06034, MSM0021622415 and MSM0021622416 from the Ministry of Education, Youth and Sports of the Czech Republic.
Návaznosti
| LC06034, projekt VaV |
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| MSM0021622415, záměr |
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| MSM0021622416, záměr |
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| 2B08068, projekt VaV |
|