HYRŠL, Pavel. Pathogenicity of four entomopathogenic nematodes species to G. mellonella larvae. Karaelmas Science and Engineering Journal. 2011, vol. 1, No 1, p. 1-6. ISSN 2146-4987.
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Basic information
Original name Pathogenicity of four entomopathogenic nematodes species to G. mellonella larvae
Name in Czech Pathogenicity of four entomopathogenic nematodes species to G. mellonella larvae
Authors HYRŠL, Pavel (203 Czech Republic, guarantor, belonging to the institution).
Edition Karaelmas Science and Engineering Journal, 2011, 2146-4987.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30102 Immunology
Country of publisher Turkey
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/11:00049819
Organization unit Faculty of Science
Keywords (in Czech) Galleria mellonella; Steinernema; Heterorhabditis; entomopathogenic nematodes; encapsulation
Keywords in English Galleria mellonella; Steinernema; Heterorhabditis; entomopathogenic nematodes; encapsulation
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 5/4/2012 13:46.
Abstract
Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In last decade they are widely used as biological control agents for pest insects of commercial crops, therefore research in this area is directly linked to agriculture. In this study, the pathogenicity of four nematode species (Heterorhabditis bacteriophora, Steinernema glaseri, Steinernema scarabaei and Steinernema feltiae) was tested against Galleria mellonella larvae. Infective stage of EPNs together with their symbiotic bacteria kills insect host within 48 hours. The results show that mortality of insect host correlates with number of invaded infective juveniles. The invasion process is very fast, IJs enters insect host within a few hours. The importance of digestive tract as entering site was clearly demonstrated, larvae with empty gut are much more sensitive to nematode infection. Nemato-bacterial complex is very effective system overcoming insect immune defences. Encapsulation as the only one cellular reaction is activated, but in very low rate and was detected only during infection of H. bacteriophora.
Links
GP206/09/P470, research and development projectName: Vliv inhibitorů biosyntézy eikosanoidů na imunitu zavíječe voskového Galleria mellonella
Investor: Czech Science Foundation, The influence of the eicosanoid biosynthesis inhibitors to immunity of the wax moth Galleria mellonella
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