Clonality assessment using 8-color flow cytometry

KOVÁŘOVÁ, Lucie, Tamara VARMUŽOVÁ, Pavla ZARBOCHOVÁ, Karthick Raja MUTHU RAJA, Renata SUSKÁ, Jana ŠTOSSOVÁ, Miroslav PENKA and Roman HÁJEK. Clonality assessment using 8-color flow cytometry. In 16th Congress of the European Hematology Association. 2011. ISSN 0390-6078.

Basic information

• Original name: Clonality assessment using 8-color flow cytometry
• Authors: KOVÁŘOVÁ, Lucie, Tamara VARMUŽOVÁ, Pavla ZARBOCHOVÁ, Karthick Raja MUTHU RAJA, Renata SUSKÁ, Jana ŠTOSSOVÁ, Miroslav PENKA and Roman HÁJEK.
• Edition: 16th Congress of the European Hematology Association, 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 6.424
• Organization unit: Faculty of Medicine
• ISSN: 0390-6078
• Keywords in English: flow cytometry; monoclonal gammopathy

Abstract

Background. Monoclonal gammopathies (MG) are characterised by presence of different numbers of clonal plasma cells (PC), lower in monoclonal gammopathy of undetermined significance (MGUS) and higher in multiple myeloma (MM). Discrimination between normal polyclonal PC (N-PC) and abnormal clonal PC (A-PC) is important in time of diagnosis and especially after treatment (transplantation etc.). Expression of surface markers CD19 and CD56 on PCs is widely used for this discrimination, but unfortunately in some cases is not enough sufficient. Aims. Verification and validation of 8-color flow cytometry protocol for clonality assessment of PC and B cell subsets in MGs.Analysis using combination of surface and intracellular markers in 8-color setting can improve detection even small clone of pathological PCs and together with B cell assessment can be very important for determination of diagnosis and monitoring after treatment.

Links

• GAP304/10/1395, research and development project: Name: Analýza klonálních progenitorů plazmatických buněk u monoklonálních gamapatií

Investor: Czech Science Foundation

• GP301/09/P457, research and development project: Name: Uplatnění multiparametrické průtokové cytometrie ve studiu patofyziologie maligních plazmatických buněk

Investor: Czech Science Foundation

• LC06027, research and development project: Name: Univerzitní výzkumné centrum - Česká myelomová skupina (Acronym: LC MGUS)

Investor: Ministry of Education, Youth and Sports of the CR, University Research Centre - Czech Myeloma Group

• MSM0021622434, plan (intention): Name: Od klasických prognostických markerů ke klinicky aplikovatelným farmakogenomickým a farmakoproteomickým projektům u mnohočetného myelomu a monoklonálních gamapatií

Investor: Ministry of Education, Youth and Sports of the CR, From classic prognostic markers to clinical applications in selected pharmacogenomic and pharmacoproteomic projects in multiple myeloma and monoclonal gammapathies

• NS10406, research and development project: Name: Vytvoření prognostického panelu u pacientů s monoklonální gamapatií nejasného významu s cílem zabránění transformace v maligní onemocnění.

Investor: Ministry of Health of the CR

• NS10408, research and development project: Name: Fenotypový profil B lymfocytů a plazmatických buněk a jeho souvislost s maligní transformací u monoklonálních gamapatií