2011
In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain
JING, Ying, Ondřej MACHOŇ, Aleš HAMPL, Petr DVOŘÁK, Ying XING et. al.Základní údaje
Originální název
In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain
Autoři
JING, Ying (156 Čína), Ondřej MACHOŇ (203 Česká republika), Aleš HAMPL (203 Česká republika, domácí), Petr DVOŘÁK (203 Česká republika, domácí), Ying XING (156 Čína) a Stefan KRAUSS (578 Norsko, garant)
Vydání
Cellular and Molecular Neurobiology, Springer, 2011, 0272-4340
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30000 3. Medical and Health Sciences
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.969
Kód RIV
RIV/00216224:14110/11:00052566
Organizační jednotka
Lékařská fakulta
UT WoS
000290914300008
Klíčová slova anglicky
Mouse; embryonic stem cell; Embryonic forebrain; Stem cell; differentiation; Wnt signaling
Příznaky
Mezinárodní význam
Změněno: 1. 8. 2013 11:03, Ing. Mgr. Věra Pospíšilíková
Anotace
V originále
Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including cortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons after embryonic day (E) 10.5. We used several cell culture protocols for differentiation of ESCs into progenitors and neurons of the dorsal forebrain. In cell culture, GFP-positive cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 10–12 day incubation. Activation of Wnt signaling during ESC differentiation further stimulated generation of D6-GFP-positive cortical cells. In contrast, differentiation protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFPpositive cells. Gene expression analysis revealed that multiple components of the canonical Wnt signaling pathway were expressed during the development of embryoid bodies. As shown by immunohistochemistry and quantitative qRT-PCR, D6-GFP-positive cells from qEBs expressed genes that are characteristic for the dorsal forebrain such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. qEBs culture allowed the formation of a D6-GFP positive pseudo-polarized neuroepithelium with the characteristic presence of N-cadherin at the apical pole resembling the structure of the developing neocortex.