Nitrate reductase whole-cell assay: side effects associated
with the use of benzyl viologen

J 2011

Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen

ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK and Igor KUČERA

Basic information

Original name

Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen

Name in Czech

Stanovení nitrátreduktasy v celých buňkách: vedlejší účinky při použití benzylviologenu

Authors

ILLICHMANOVÁ, Adéla (203 Czech Republic, belonging to the institution), Miroslava JANŮ (203 Czech Republic, belonging to the institution), Vojtěch SEDLÁČEK (203 Czech Republic, belonging to the institution) and Igor KUČERA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Folia Microbiologica, Dordrecht, The Netherlands, Springer, 2011, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.677

RIV identification code

RIV/00216224:14310/11:00052814

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s12223-011-0004-6

UT WoS

000290451500012

Keywords (in Czech)

nitrátreduktasa; umělý elektronový donor; transport dusičnanu; inhibice

Keywords in English

nitrate reductase; artificial electron donor; nitrate transport; inhibition

Abstract

V originále

A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK and Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. Folia Microbiologica. Dordrecht, The Netherlands: Springer, 2011, vol. 56, No 1, p. 72-76. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-011-0004-6.

@article{947101,
   author = {Illichmanová, Adéla and Janů, Miroslava and Sedláček, Vojtěch and Kučera, Igor},
   article_location = {Dordrecht, The Netherlands},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s12223-011-0004-6},
   keywords = {nitrate reductase; artificial electron donor; nitrate transport; inhibition},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen},
   url = {http://www.springerlink.com/content/u876462762641134/},
   volume = {56},
   year = {2011}
}

TY  - JOUR
ID  - 947101
AU  - Illichmanová, Adéla - Janů, Miroslava - Sedláček, Vojtěch - Kučera, Igor
PY  - 2011
TI  - Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen
JF  - Folia Microbiologica
VL  - 56
IS  - 1
SP  - 72-76
EP  - 72-76
PB  - Springer
SN  - 00155632
KW  - nitrate reductase
KW  - artificial electron donor
KW  - nitrate transport
KW  - inhibition
UR  - http://www.springerlink.com/content/u876462762641134/
N2  - A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.
ER  -

ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK and Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. \textit{Folia Microbiologica}. Dordrecht, The Netherlands: Springer, 2011, vol.~56, No~1, p.~72-76. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-011-0004-6.

Detailed Information on Publication Record