Nitrate reductase whole-cell assay: side effects associated
with the use of benzyl viologen

J 2011

Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen

ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK a Igor KUČERA

Základní údaje

Originální název

Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen

Název česky

Stanovení nitrátreduktasy v celých buňkách: vedlejší účinky při použití benzylviologenu

Autoři

ILLICHMANOVÁ, Adéla (203 Česká republika, domácí), Miroslava JANŮ (203 Česká republika, domácí), Vojtěch SEDLÁČEK (203 Česká republika, domácí) a Igor KUČERA (203 Česká republika, garant, domácí)

Vydání

Folia Microbiologica, Dordrecht, The Netherlands, Springer, 2011, 0015-5632

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.677

Kód RIV

RIV/00216224:14310/11:00052814

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s12223-011-0004-6

UT WoS

000290451500012

Klíčová slova česky

nitrátreduktasa; umělý elektronový donor; transport dusičnanu; inhibice

Klíčová slova anglicky

nitrate reductase; artificial electron donor; nitrate transport; inhibition

Štítky

AKR, rivok

Změněno: 12. 4. 2012 12:31, Ing. Andrea Mikešková

Anotace

V originále

A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK a Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. Folia Microbiologica. Dordrecht, The Netherlands: Springer, 2011, roč. 56, č. 1, s. 72-76. ISSN 0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-011-0004-6.

@article{947101,
   author = {Illichmanová, Adéla and Janů, Miroslava and Sedláček, Vojtěch and Kučera, Igor},
   article_location = {Dordrecht, The Netherlands},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s12223-011-0004-6},
   keywords = {nitrate reductase; artificial electron donor; nitrate transport; inhibition},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen},
   url = {http://www.springerlink.com/content/u876462762641134/},
   volume = {56},
   year = {2011}
}

TY  - JOUR
ID  - 947101
AU  - Illichmanová, Adéla - Janů, Miroslava - Sedláček, Vojtěch - Kučera, Igor
PY  - 2011
TI  - Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen
JF  - Folia Microbiologica
VL  - 56
IS  - 1
SP  - 72-76
EP  - 72-76
PB  - Springer
SN  - 00155632
KW  - nitrate reductase
KW  - artificial electron donor
KW  - nitrate transport
KW  - inhibition
UR  - http://www.springerlink.com/content/u876462762641134/
N2  - A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.
ER  -

ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK a Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. \textit{Folia Microbiologica}. Dordrecht, The Netherlands: Springer, 2011, roč.~56, č.~1, s.~72-76. ISSN~0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-011-0004-6.

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