Heterogeneity in the kinetics of nuclear proteins and
trajectories of substructures associated with heterochromatin

J 2011

Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin

STIXOVÁ, Lenka, Eva BÁRTOVÁ, Pavel MATULA, Ondřej DANĚK, Soňa LEGARTOVÁ et. al.

Základní údaje

Originální název

Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin

Autoři

STIXOVÁ, Lenka (203 Česká republika), Eva BÁRTOVÁ (203 Česká republika), Pavel MATULA (203 Česká republika, garant, domácí), Ondřej DANĚK (203 Česká republika, domácí), Soňa LEGARTOVÁ (703 Slovensko) a Stanislav KOZUBEK (203 Česká republika)

Vydání

Epigenetics & Chromatin, 2011, 1756-8935

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.462

Kód RIV

RIV/00216224:14330/11:00052815

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1186/1756-8935-4-5

UT WoS

000288995300001

Klíčová slova anglicky

protein dynamics HP1b BMI1 TRF1 trajectory heterochromatin

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 4. 2012 00:09, doc. RNDr. Pavel Matula, Ph.D.

Anotace

V originále

We show that protein dynamics are influenced by many factors and events, including nuclear pattern and transcription activity. A slower recovery after photobleaching was found when proteins, such as HP1b, BMI1, TRF1, and others accumulated at specific foci. In identical cells, proteins that were evenly dispersed throughout the nucleoplasm recovered more rapidly. Distinct trajectories for HP1b, BMI1, and TRF1 were observed after hyperacetylation or suppression of transcription. The relationship between protein trajectory and transcription level was confirmed for telomeric protein TRF1, but not for HP1b or BMI1 proteins. Moreover, heterogeneity of foci movement was especially observed when we made distinctions between centrally and peripherally positioned foci. Based on our results, we propose that protein kinetics are likely influenced by several factors, including chromatin condensation, differentiation, local protein density, protein binding efficiency, and nuclear pattern. These factors and events likely cooperate to dictate the mobility of particular proteins.

Návaznosti

LC535, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii

Citovat

STIXOVÁ, Lenka, Eva BÁRTOVÁ, Pavel MATULA, Ondřej DANĚK, Soňa LEGARTOVÁ a Stanislav KOZUBEK. Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin. Epigenetics & Chromatin. 2011, roč. 4, č. 5, s. 1-17. ISSN 1756-8935. Dostupné z: https://dx.doi.org/10.1186/1756-8935-4-5.

@article{947102,
   author = {Stixová, Lenka and Bártová, Eva and Matula, Pavel and Daněk, Ondřej and Legartová, Soňa and Kozubek, Stanislav},
   article_number = {5},
   doi = {http://dx.doi.org/10.1186/1756-8935-4-5},
   keywords = {protein dynamics HP1b BMI1 TRF1 trajectory heterochromatin},
   language = {eng},
   issn = {1756-8935},
   journal = {Epigenetics & Chromatin},
   title = {Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin},
   url = {http://www.epigeneticsandchromatin.com/content/4/1/5},
   volume = {4},
   year = {2011}
}

TY  - JOUR
ID  - 947102
AU  - Stixová, Lenka - Bártová, Eva - Matula, Pavel - Daněk, Ondřej - Legartová, Soňa - Kozubek, Stanislav
PY  - 2011
TI  - Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin
JF  - Epigenetics & Chromatin
VL  - 4
IS  - 5
SP  - 1-17
EP  - 1-17
SN  - 17568935
KW  - protein dynamics HP1b BMI1 TRF1 trajectory heterochromatin
UR  - http://www.epigeneticsandchromatin.com/content/4/1/5
L2  - http://www.epigeneticsandchromatin.com/content/4/1/5
N2  - We show that protein dynamics are influenced by many factors and events, including nuclear pattern and transcription activity. A slower recovery after photobleaching was found when proteins, such as HP1b, BMI1, TRF1, and others accumulated at specific foci. In identical cells, proteins that were evenly dispersed throughout the nucleoplasm recovered more rapidly. Distinct trajectories for HP1b, BMI1, and TRF1 were observed after hyperacetylation or suppression of transcription. The relationship between protein trajectory and transcription level was confirmed for telomeric protein TRF1, but not for HP1b or BMI1 proteins. Moreover, heterogeneity of foci movement was especially observed when we made distinctions between centrally and peripherally positioned foci. Based on our results, we propose that protein kinetics are likely influenced by several factors, including chromatin condensation, differentiation, local protein density, protein binding efficiency, and nuclear pattern. These factors and events likely cooperate to dictate the mobility of particular proteins.
ER  -

STIXOVÁ, Lenka, Eva BÁRTOVÁ, Pavel MATULA, Ondřej DANĚK, Soňa LEGARTOVÁ a Stanislav KOZUBEK. Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin. \textit{Epigenetics \&{} Chromatin}. 2011, roč.~4, č.~5, s.~1-17. ISSN~1756-8935. Dostupné z: https://dx.doi.org/10.1186/1756-8935-4-5.

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