| KOUDELÁKOVÁ, Táňa, Eva CHOVANCOVÁ, Jan BREZOVSKÝ, Marta MONINCOVÁ, Andrea FOŘTOVÁ, Jiří JARKOVSKÝ a Jiří DAMBORSKÝ. Substrate Specificity of Haloalkane Dehalogenases. Biochemical Journal. 2011, roč. 435, č. 435, s. 345-354. ISSN 0264-6021. Dostupné z: https://dx.doi.org/10.1042/BJ20101405. |
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@article{947495,
author = {Koudeláková, Táňa and Chovancová, Eva and Brezovský, Jan and Monincová, Marta and Fořtová, Andrea and Jarkovský, Jiří and Damborský, Jiří},
article_number = {435},
doi = {http://dx.doi.org/10.1042/BJ20101405},
keywords = {substrate specificity; haloalkane dehalogenases; halogenated compounds},
language = {eng},
issn = {0264-6021},
journal = {Biochemical Journal},
title = {Substrate Specificity of Haloalkane Dehalogenases},
volume = {435},
year = {2011}
}
TY - JOUR
ID - 947495
AU - Koudeláková, Táňa - Chovancová, Eva - Brezovský, Jan - Monincová, Marta - Fořtová, Andrea - Jarkovský, Jiří - Damborský, Jiří
PY - 2011
TI - Substrate Specificity of Haloalkane Dehalogenases
JF - Biochemical Journal
VL - 435
IS - 435
SP - 345-354
EP - 345-354
SN - 02646021
KW - substrate specificity
KW - haloalkane dehalogenases
KW - halogenated compounds
N2 - An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate specificity data, and analysis using multivariate statistics. We describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. The enzymes were characterized experimentally using a set of 30 substrates selected using statistical experimental design from a set of nearly 200 halogenated compounds.
ER -
KOUDELÁKOVÁ, Táňa, Eva CHOVANCOVÁ, Jan BREZOVSKÝ, Marta MONINCOVÁ, Andrea FOŘTOVÁ, Jiří JARKOVSKÝ a Jiří DAMBORSKÝ. Substrate Specificity of Haloalkane Dehalogenases. \textit{Biochemical Journal}. 2011, roč.~435, č.~435, s.~345-354. ISSN~0264-6021. Dostupné z: https://dx.doi.org/10.1042/BJ20101405.
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