| HASAN, Khomaini, Andrea FOŘTOVÁ, Táňa KOUDELÁKOVÁ, Radka CHALOUPKOVÁ, M. ISHITSUKA, Yuji NAGATA, Jiří DAMBORSKÝ a Zbyněk PROKOP. Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58. Applied and Environmental Microbiology. 2011, roč. 77, č. 5, s. 1881-1884. ISSN 0099-2240. Dostupné z: https://dx.doi.org/10.1128/AEM.02109-10. |
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@article{947515,
author = {Hasan, Khomaini and Fořtová, Andrea and Koudeláková, Táňa and Chaloupková, Radka and Ishitsuka, M. and Nagata, Yuji and Damborský, Jiří and Prokop, Zbyněk},
article_number = {5},
doi = {http://dx.doi.org/10.1128/AEM.02109-10},
keywords = {Novel Haloalkane Dehalogenase DatA ;Agrobacterium tumefaciens C58},
language = {eng},
issn = {0099-2240},
journal = {Applied and Environmental Microbiology},
title = {Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.},
volume = {77},
year = {2011}
}
TY - JOUR
ID - 947515
AU - Hasan, Khomaini - Fořtová, Andrea - Koudeláková, Táňa - Chaloupková, Radka - Ishitsuka, M. - Nagata, Yuji - Damborský, Jiří - Prokop, Zbyněk
PY - 2011
TI - Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.
JF - Applied and Environmental Microbiology
VL - 77
IS - 5
SP - 1881-1884
EP - 1881-1884
SN - 00992240
KW - Novel Haloalkane Dehalogenase DatA ;Agrobacterium tumefaciens C58
N2 - Abstract: We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters.
ER -
HASAN, Khomaini, Andrea FOŘTOVÁ, Táňa KOUDELÁKOVÁ, Radka CHALOUPKOVÁ, M. ISHITSUKA, Yuji NAGATA, Jiří DAMBORSKÝ a Zbyněk PROKOP. Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58. \textit{Applied and Environmental Microbiology}. 2011, roč.~77, č.~5, s.~1881-1884. ISSN~0099-2240. Dostupné z: https://dx.doi.org/10.1128/AEM.02109-10.
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