Digital holographic microscopy in human sperm imaging

J 2011

Digital holographic microscopy in human sperm imaging

CRHA, Igor, Jana ŽÁKOVÁ, Martin HUSER, Pavel VENTRUBA, Eva LOUSOVÁ et. al.

Základní údaje

Originální název

Digital holographic microscopy in human sperm imaging

Autoři

CRHA, Igor (203 Česká republika, garant, domácí), Jana ŽÁKOVÁ (203 Česká republika, domácí), Martin HUSER (203 Česká republika, domácí), Pavel VENTRUBA (203 Česká republika, domácí), Eva LOUSOVÁ (203 Česká republika) a Michal POHANKA (203 Česká republika, domácí)

Vydání

Journal of Assisted Reproduction and Genetics, 2011, 1058-0468

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.844

Kód RIV

RIV/00216224:14110/11:00056979

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s10815-011-9584-y

UT WoS

000294734900011

Klíčová slova anglicky

Digital holographic microscopy; Sperm imaging; Spermatozoon; Male infertility; Chromatin integrity

Příznaky

Mezinárodní význam

Změněno: 23. 3. 2012 13:39, Mgr. Michal Petr

Anotace

V originále

The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT). DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested. In NZ samples median phase shifts were in the range 2.72–3.21 rad and 2.00–2.15 in OAT samples. Differences among individual samples were statistically significant (p<0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p<0.001). Quantitative evaluation of the phase shift by DHM could provide new information on the exact structure and composition of the sperm head. At present, this technique is not established for clinical utility.

Návaznosti

NS9661, projekt VaV

Název: Vliv metabolismu homocysteinu na poruchy plodnosti.

Investor: Ministerstvo zdravotnictví ČR, Vliv metabolismu homocysteinu na poruchy plodnosti

Citovat

CRHA, Igor, Jana ŽÁKOVÁ, Martin HUSER, Pavel VENTRUBA, Eva LOUSOVÁ a Michal POHANKA. Digital holographic microscopy in human sperm imaging. Journal of Assisted Reproduction and Genetics. 2011, roč. 28, č. 8, s. 725-729. ISSN 1058-0468. Dostupné z: https://dx.doi.org/10.1007/s10815-011-9584-y.

@article{950024,
   author = {Crha, Igor and Žáková, Jana and Huser, Martin and Ventruba, Pavel and Lousová, Eva and Pohanka, Michal},
   article_number = {8},
   doi = {http://dx.doi.org/10.1007/s10815-011-9584-y},
   keywords = {Digital holographic microscopy; Sperm imaging; Spermatozoon; Male infertility; Chromatin integrity},
   language = {eng},
   issn = {1058-0468},
   journal = {Journal of Assisted Reproduction and Genetics},
   title = {Digital holographic microscopy in human sperm imaging},
   volume = {28},
   year = {2011}
}

TY  - JOUR
ID  - 950024
AU  - Crha, Igor - Žáková, Jana - Huser, Martin - Ventruba, Pavel - Lousová, Eva - Pohanka, Michal
PY  - 2011
TI  - Digital holographic microscopy in human sperm imaging
JF  - Journal of Assisted Reproduction and Genetics
VL  - 28
IS  - 8
SP  - 725-729
EP  - 725-729
SN  - 10580468
KW  - Digital holographic microscopy
KW  - Sperm imaging
KW  - Spermatozoon
KW  - Male infertility
KW  - Chromatin integrity
N2  - The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT). DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested. In NZ samples median phase shifts were in the range 2.72–3.21 rad and 2.00–2.15 in OAT samples. Differences among individual samples were statistically significant (p<0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p<0.001). Quantitative evaluation of the phase shift by DHM could provide new information on the exact structure and composition of the sperm head. At present, this technique is not established for clinical utility.
ER  -

CRHA, Igor, Jana ŽÁKOVÁ, Martin HUSER, Pavel VENTRUBA, Eva LOUSOVÁ a Michal POHANKA. Digital holographic microscopy in human sperm imaging. \textit{Journal of Assisted Reproduction and Genetics}. 2011, roč.~28, č.~8, s.~725-729. ISSN~1058-0468. Dostupné z: https://dx.doi.org/10.1007/s10815-011-9584-y.

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