| CRHA, Igor, Jana ŽÁKOVÁ, Martin HUSER, Pavel VENTRUBA, Eva LOUSOVÁ a Michal POHANKA. Digital holographic microscopy in human sperm imaging. Journal of Assisted Reproduction and Genetics. 2011, roč. 28, č. 8, s. 725-729. ISSN 1058-0468. Dostupné z: https://dx.doi.org/10.1007/s10815-011-9584-y. |
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@article{950024,
author = {Crha, Igor and Žáková, Jana and Huser, Martin and Ventruba, Pavel and Lousová, Eva and Pohanka, Michal},
article_number = {8},
doi = {http://dx.doi.org/10.1007/s10815-011-9584-y},
keywords = {Digital holographic microscopy; Sperm imaging; Spermatozoon; Male infertility; Chromatin integrity},
language = {eng},
issn = {1058-0468},
journal = {Journal of Assisted Reproduction and Genetics},
title = {Digital holographic microscopy in human sperm imaging},
volume = {28},
year = {2011}
}
TY - JOUR
ID - 950024
AU - Crha, Igor - Žáková, Jana - Huser, Martin - Ventruba, Pavel - Lousová, Eva - Pohanka, Michal
PY - 2011
TI - Digital holographic microscopy in human sperm imaging
JF - Journal of Assisted Reproduction and Genetics
VL - 28
IS - 8
SP - 725-729
EP - 725-729
SN - 10580468
KW - Digital holographic microscopy
KW - Sperm imaging
KW - Spermatozoon
KW - Male infertility
KW - Chromatin integrity
N2 - The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT). DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested. In NZ samples median phase shifts were in the range 2.72–3.21 rad and 2.00–2.15 in OAT samples. Differences among individual samples were statistically significant (p<0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p<0.001). Quantitative evaluation of the phase shift by DHM could provide new information on the exact structure and composition of the sperm head. At present, this technique is not established for clinical utility.
ER -
CRHA, Igor, Jana ŽÁKOVÁ, Martin HUSER, Pavel VENTRUBA, Eva LOUSOVÁ a Michal POHANKA. Digital holographic microscopy in human sperm imaging. \textit{Journal of Assisted Reproduction and Genetics}. 2011, roč.~28, č.~8, s.~725-729. ISSN~1058-0468. Dostupné z: https://dx.doi.org/10.1007/s10815-011-9584-y.
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